Epstein-Barr Virus LMP2A Antibody | 14B7

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Epstein-Barr Virus LMP2A Antibody | 14B7 gallery image 1

Published customer image:
Rat anti Epstein-Barr Virus LMP2A antibody, clone 14B7 (MCA2466) used for the detection of LMP2A in cell extracts by western blotting. Epstein-Barr virus nuclear antigen (EBNA1) is detected using Mouse anti EBNA1 antibody, clone E1-2.5 (MCA2707).
Image caption:
(a) Epitope comparison amongst the variants and those observed in the 3 cell lines studied. Red letters denote amino acid mutation. (b) Quantitation of HLA-A0201/LMP1,/LMP2A and/EBNA1 on CCRF-SB, RPMI-6666 and C666-1A2 using TCR-like mAbs based on flow cytometry. Error bars are standard deviation from an average of three independent experiments. **p<0.01. n.d. denotes not detectable. (c) LMP1, LMP2A and EBNA1 in each of the cell lines was determined using RT-PCR and Western blot. β-actin was used as control.

From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA.
Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.
Sci Rep. 2013 Nov 18;3:3232.

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Epstein-Barr Virus LMP2A Antibody | 14B7 gallery image 2

Published customer image:
Rat anti Epstein-Barr Virus LMP2A antibody, clone 14B7 (MCA2466) used for the detection of LMP2A in cell extracts by Immunofluorescence and immunohistochemistry. Epstein-Barr virus nuclear antigen (EBNA1) is detected using Mouse anti EBNA1 antibody, clone E1-2.5 (MCA2707).
Image caption:
Generation of Hu-NSG mice EBV model and staining of infected mouse spleen and clinical NPC biopsies with TCR-like mAbs.
(a) Experimental workflow of the Hu-NSG mice EBV model -isolation of HSC; injection into NSG mice; flow cytometric analysis of reconstitution; EBV infection of Hu-NSG mice; and development of splenic lesions. The reconsitution of the Hu-NSG mice with human immune cells was approximately 65% ± 3.9%. (b) Spleens of EBV infected HLA-A0201 Hu-NSG mice showed positive red staining for TCR-like mAbs specific to LMP1, LMP2A and EBNA1 binding. Scale bars, 50 μm. (c) Immunological staining of NPC biopsy. Control panel was stained with secondary antibody. BB7.2 antibody stained for HLA-A02. The other two panels were stained with anti-HLA-A0201/LMP2A and anti-HLA-A0201/EBNA1 mAb. Positive staining was observed to be reddish brown. Scale bars, 25 μm.

From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA.
Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.
Sci Rep. 2013 Nov 18;3:3232.

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  • Rat anti Epstein-Barr Virus LMP2A
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    14B7
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2466IF, IP, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA2466
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Epstein-Barr Virus LMP2A antibody, clone 14B7 recognizes latent membrane protein 2A (LMP2A) of Epstein-Barr virus (EBV). EBV is a human herpesvirus, which is associated with conditions such as Hodgkin’s disease and Burkitt’s Lymphoma and is the causative agent in mononucleosis in adolescents.

      EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases.

      Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals.

      Rat anti Epstein-Barr Virus LMP2A antibody, clone 14B7 detects wild-type LMP2A protein.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Bacterial TrpE-LMP2A fusion protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Lou/C rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting1/1001/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Epstein-Barr Virus LMP2A Antibody Formats

    Formats Clone Applications Sizes available
    Epstein-Barr Virus LMP2A Antibody : Purified 14B7 IF, IP, WB 0.25 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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