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Dengue Virus Type 2 NS1 Antigen

Recombinant Dengue Virus Type 2 NS1 Antigen

Product Type
Recombinant Protein
Specificity
Dengue Virus Type 2 NS1 Antigen

Product Code Applications Pack Size List Price Your Price Qty
PIP048A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 µg loader
List Price Your Price
loader
PIP048B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 µg loader
List Price Your Price
loader

Recombinant dengue virus type 2 NS1 antigen is a purified preparation of the dengue virus serotype 2 (DENV2), non-structural protein 1 (NS1). DENV2 is one of four (DENV1-4) antigenically distinct, closely related viral serotypes belonging to the Flaviviridae family, genus flavivirus. All four serotypes are known to cause viral infection in humans.

Dengue virus is common mosquito-borne infection and a major cause of morbidity in tropical and subtropical regions. There is currently no vaccine to prevent, or effective anti-viral drugs to treat, dengue virus infection. In many cases infection is asymptomatic and the majority of individuals who get ill only suffer the mild, non-specific febrile symptoms characteristic of dengue fever (DF). Only a minority of infections result in severe disease, manifesting as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue virus infection gives lifelong immunity to the serotype in question but subsequent infection with another serotype may increase the likelihood of severe disease.

The NS1 glycoprotein is essential for viral replication and viability, and since this protein is secreted into the bloodstream, tests have been developed to diagnose DENV infections using NS1, including antigen-capture ELISA, lateral flow antigen detection, and the measurement of NS1-specific IgM and IgG responses (Guzman, M.G. et al. 2010)).

Recombinant DENV2 NS1 antigen is presented in its native folded state complete with post-translational modifications, delivering optimal antigenicity and making it suitable for use in vaccine research and serology-based assays.

Target Species
Viral
Product Form
Purified recombinant protein with a C-terminal 6 x His-tag - liquid
Preparation
Recombinant dengue virus serotype 2 NS1 protein, sequence strain Thailand/16681/84, expressed in 293 human cells
Source
HEK-293
Buffer Solution
Dulbecco's phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Approx. Protein Concentrations
PIP048A: Current, batch-specific concentration 0.60 mg/ml
PIP048B: Current, batch-specific concentration 0.87 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.

Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Further Reading

  1. Guzman, M.G. et al. (2010) Dengue: a continuing global threat.
    Nat Rev Microbiol. 8 (12 Suppl): S7-16.

Synonyms
DENV2 NS1 Antigen

PIP048A

154667 158148 165348 165865 165906 180209

PIP048B

150350 161980

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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