Dengue Virus Type 2

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  • Native Dengue Virus Type 2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Antigen
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP006Edatasheet pdfdatasheet pdf1 ml
    PIP006
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Native Dengue Virus Type 2 is a preparation of viral particles concentrated from tissue culture supernatant. Dengue Virus Type 2 is one of four antigenically distinct yet closely related viral serotypes belonging to the family Flaviviridae known to cause Dengue fever in humans. Dengue infection is a major cause of morbidity in tropical and subtropical regions. It is a mosquito borne viral infection that may be asymptomatic or may cause undifferentiated fever, dengue fever, dengue haemorrhagic fever or dengue shock syndrome. Currently, there is no vaccine or effective antiviral drugs to treat the infection.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Inactivated, highly purified preparation of Dengue virus type 2 particles - liquid.
    • Reconstitution
    • Preparation
      Dengue virus particles are concentrated from tissue culture supernatant by precipitation and ultracentrifugation. Antigen is purified by sucrose density gradient centrifugation. Virus particles are separated from the sucrose containing buffer by ultracentrifugation before resuspension in Medium 199.

      The antigen preparation is inactivated by room temperature incubation with formaldehyde. The formaldehyde is neutralised by the addition of sodium bisulphite.
    • Source
      Dengue virus type 2 strain 16681 cultured in Vero cells.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.60 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Medium 199
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Dengue Virus Type 2 Formats

    Formats Applications Sizes available
    Dengue Virus Type 2 : Inactivated Pathogen E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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