Dengue Virus antibody | Dengue 1-11(3)
Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) used for the evaluation of dengue viral load by capture ELISA.
Image caption:
Anti-DIII sera recognise viral E protein. (A) Immunofluorescence of Vero cells infected with DENV of each serotype, reacted with the serotype-specific anti-DIII pools of sera (top row), the negative control sera (ctrl., middle row) and mAb 4G2 (bottom row). In each case, non-infected cells (n.i., rightmost column) were also used as controls. Bars represent 50 μm. (B) ELISA on whole infective viral particles. Anti-DIII serotype-specific pools diluted to 100 ng/ml and sera from mock-immunised animals were used onto virus particles captured on plates coated with a human serum reactive against all four serotypes. mAb Dengue 1–11 reactive against DENV1 E (at 1 μg/ml) and a Dengue pan-reactive serum against all four serotypes were used as positive controls.
From: Poggianella M, Slon Campos JL, Chan KR, Tan HC, Bestagno M, Ooi EE, et al. (2015)
Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.
PLoS Negl Trop Dis 9(7): e0003947.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) (MCA2277) used to detect dengue viral protein in dengie-poly IgG scaffold by western blotting.
Image caption:
Expression, purification and molecular fractionation of dengue poly‐immunoglobulin G scaffold (D‐PIGS). (a) D‐PIGS were expressed in Nicotiana benthamiana plants and the extracts analysed by Western blotting under reducing (R) or nonreducing (NR) conditions using antidengue or anti‐Fc gamma antibodies. ‘PC’ is the positive control (recombinant cEDIII or human IgG1, respectively). Lane 1: wild‐type plant extract; lane 2: unfractionated D‐PIGS. Positions of the single chain (S), monomer (M) and polymers (P) are indicated.
From: Kim MY, Copland A, Nayak K, Chandele A, Ahmed MS, Zhang Q, Diogo GR, Paul MJ, Hofmann S, Yang MS, Jang YS, Ma JK, Reljic R.
Plant-expressed Fc-fusion protein tetravalent dengue vaccine with inherent adjuvant properties.
Plant Biotechnol J. 2018 Jul;16(7):1283-94.
doi: 10.1111/pbi.12869.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dengue Virus
- Product Type
- Monoclonal Antibody
- Clone
- Dengue 1-11(3)
- Isotype
- IgG2a
- Specificity
- Dengue Virus
Antibody Quality and Performance Guarantee
| Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus. Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4. The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunofluorescence | |
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| Immunohistology - Paraffin 1 | |
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| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- This antibody detects an anti-E/envelope specific band of ~61 kDa under reducing conditions. A weak secondary band of ~80 kDa may also be apparent.
References for Dengue Virus antibody
-
Kim,T.G. et al. (2010) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants.
Protein Expr Purif. 74:236-41. -
Nguyen, N.L. et al. (2015) Expression and characterization of an M cell-specific ligand-fused dengue virus tetravalent epitope using Saccharomyces cerevisiae.
J Biosci Bioeng. 119 (1): 19-27. -
Poggianella, M. et al. (2015) Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.
PLoS Negl Trop Dis. 9 (7): e0003947. -
Milic, N.L. et el. (2015) Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus).
Dev Comp Immunol. 51 (1): 108-15. -
Slon Campos, J.L. (2017) Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
PhD thesis The Open University. -
Kim, M.Y. et al. (2018) Plant-expressed Fc-fusion protein tetravalent dengue vaccine with inherent adjuvant properties.
Plant Biotechnol J. 16 (7): 1283-94. -
Kim, B. & Kim, M. (2019) Evaluation of the oral immunogenicity of M cell-targeted tetravalent EDIII antigen for development of plant-based edible vaccine against dengue infection
Plant Cell, Tissue and Organ Culture (PCTOC). 137 (1): 1-10. -
Kim, M.Y. et al. (2024) Marked enhancement of the immunogenicity of plant-expressed IgG-Fc fusion proteins by inclusion of cholera toxin non-toxic B subunit within the single polypeptide.
Plant Biotechnol J. Jan 01 [Epub ahead of print].
View The Latest Product References
Further Reading
-
Gubler, D.J. (1998) Dengue and dengue hemorrhagic fever.
Clin Microbiol Rev. 11 (3): 480-96. -
Loroño-pino, M.A. et al. (1999) Common occurrence of concurrent infections by multiple dengue virus serotypes.
Am J Trop Med Hyg. 61 (5): 725-30. -
PhilipSamuel, P. & Tyagi, B.K. (2006) Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes.
Indian J Med Res. 123 (5): 615-28.
- RRID
- AB_609623
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