Vancomycin Antibody

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Published customer image:
Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by western blotting
Image caption:
Hapten evaluation. a Ponceau S staining indicates protein presence in the coupled haptens. b Immunoblotting indicates corresponding antibiotic presence in both coupled haptens specifically.

From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.
SpringerPlus 2015, 4:614

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Vancomycin Antibody gallery image 2

Published customer image:
Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISA
Image caption:
Coating optimization. a The optimal concentration of the gentamicin-BSA hapten was based on the pattern of the calibration curve, which indicated a concentration dependant absorbance. b The optimal concentration of the vancomycin-BSA hapten was determined on the relation between coating concentration and absorbance level.

From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.
SpringerPlus 2015, 4:614

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Vancomycin Antibody gallery image 3

Published customer image:
Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISA
Image caption:
Specificity and sensitivity of the antibiotic ELISAs. a The influence of vancomycin on the gentamicin ELISA. b The influence of gentamicin on the vancomycin ELISA. c The validation of the calibration curve of the gentamicin ELISA. Lines indicate upper and lower 10 % range of the calibration curve. d The validation of the calibration curve of the vancomycin ELISA. Lines indicate upper and lower 10 % range of the calibration curve. Error bars indicate standard deviation.

From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.
SpringerPlus 2015, 4:614

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Vancomycin Antibody gallery image 4

Published customer image:
Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISA
Image caption:
ELISA for gentamicin and vancomycin. A combined hapten of both gentamicin and vancomycin was used to coat wells for the detection of either gentamicin (black circles) or vancomycin (black squares) in a calibrations series of the respective antibiotics. Error bars indicate standard deviation.

From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.
SpringerPlus 2015, 4:614

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Vancomycin Antibody gallery image 5

Published customer image:
Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISA
Image caption:
Schematic representation of the antibiotic ELISA procedure and detection. The antibiotic-BSA hapten is coated to a microtiter plate. An antibody directed against the antibiotic compound can interact with either the antibiotic in the sample or the antibiotic-hapten coated to the microtiter plate. After washing only the microtiter plate-bound antibodies remain present and can be detected by HRP-conjugated secondary antibodies in combination with TMB (colorimetric detection at 450 nm). A high concentration of antibiotics in a sample will lead to more interaction with an antibiotic-specific antibody in solution and thus result in less bound antibodies to the microtiter plate leading to a low signal when detected at 450 nm. This indicates that the measured signal is inversely correlated with the concentration of antibiotic in the measured sample. Samples with a known concentration of antibiotics can be used for a calibration curve; regression from this curve will allow calculation of the antibiotic concentration in unknown samples.

From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.
SpringerPlus 2015, 4:614

Enlarge
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  • Rabbit anti Vancomycin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9520-0304E, WBdatasheet pdfdatasheet pdf1 ml
    9520-0304
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Vancomycin antibody recognizes vancomycin, a glycopeptide antibiotic derived from the actinobacterium Amycolatopsis orientalis that acts by inhibiting cell wall biosynthesis and altering the permeability of the bacterial cell membrane (Cheung & DiPiro 1986) .

      Vancomycin is regarded as an antibiotic of last resort as in high doses it can be toxic to the ears (Lackner 1984) and kidneys (Hanrahan et al.2015), whilst in low doses hypersensitivity reactions may occur (Blumenthal et al. 2015).

      Rabbit anti Vancomycin antibody has been used for the development of a competitive ELISA for the detection of vancomycin in protein containing samples (Oderkerken et al. 2015)
    • Intended Use
    • Target Species
      Chemical
    • Product Form
      Purified IgG - liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Vancomycin conjugated to Keyhole limpet Haemocyanin plus thyroglobulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Vancomycin Antibody Formats

    Formats Applications Sizes available
    Vancomycin Antibody : Purified E, WB 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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