Ustekinumab Antibody | 17829-hIgG1

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Ustekinumab Antibody | 17829-hIgG1 gallery image 1

ELISA demonstrating the specificity of HCA211 using various antigens as coating reagents (A: Human serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Bevacizumab, E: Adalimumab, F: Alemtuzumab, G: Infliximab, H: Ustekinumab) and HCA211 conjugated to HRP as the detection reagent.
Antigens were coated at 5 µg/mL on a microtiter plate overnight. After washing and blocking with 5% milk powder in PBST, HRP-conjugated HCA211 (20µL from a 2µg/mL solution in HiSPEC buffer,(BUF049) was added. Detection was performed using the QuantaBlu™ fluorogenic peroxidase substrate

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Human anti ustekinumab bridging ELISA for Anti-Drug Antibody (ADA) assay development

A microtitre plate was coated over night with ustekinumab at a concentration of 1.5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human anti ustekinumab. Detection was performed using HRP-conjugated ustekinumab at a concentration of 4 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of two measurements

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  • Human anti Ustekinumab
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    Anti-ustekinumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human immunoglobulin format; it is suitable as a reference standard in an immune response assay to measure anti-drug antibody levels for ustekinumab or biosimilars.
    • Product Type
      Monoclonal Antibody
    • Clone
      17829-hIgG1
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA211Edatasheet pdfdatasheet pdf0.1 mg
      HCA211
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Ustekinumab (branded as Stelara®) is a fully human monoclonal antibody drug (IgG1/kappa) that has been approved for treatment of moderate to severe psoriasis. Ustekinumab is also being investigated for the treatment of psoriatic arthritis, for multiple sclerosis and sarcoidosis. Studies indicate that it may also have potential for treating severe Crohns disease.
        Ustekinumab is directed against the p40 subunit of interleukin-12 and interleukin-23 and acts by blocking the binding of these two cytokines to, and preventing activation of, T-lymphocytes.

        Clone 17829-hIgG is an anti-idiotypic antibody that specifically recognises the Ustekinumab monoclonal antibody and inhibits the binding of Ustekinumab to its target. It can be used to measure Ustekinumab levels in serum from patients.

        The monovalent intrinsic affinity of this antibody was measured as KD=0.28 nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Ustekinumab.

        For our full range of Ustekinumab antibodies, please visit our website.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Ustekinumab
      • Purity
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Stelara is a registered trademark of Centocor.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or as a detection antibody when conjugated to HRP in a bridging ELISA together with AbD18334 (HCA209) as the capture reagent. Conjugation of this antibody to HRP can be undertaken using a Lynx Rapid HRP conjugation kit, these kits may be purchased from Bio-Rad.
        Protocol: PK bridging ELISA to measure free drug
        Protocol: ADA bridging ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Ustekinumab Antibody Formats

      Formats Clone Applications Sizes available
      Ustekinumab Antibody : Purified 17829-hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK005P1 Conjugation For 20mg AntibodyCJ
            LNK005P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            LYNX Rapid HRP Antibody Conjugation KitLNK003P1 Conjugation For 4mg AntibodyCJ
            LNK003P
            LYNX Rapid HRP Antibody Conjugation KitLNK006P3 Conjugations For 40µg AntibodyCJ
            LNK006P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            LYNX Rapid HRP Antibody Conjugation KitLNK004P5 Conjugations For 4mg AntibodyCJ
            LNK004P

            Recommended Positive Controls

              Histology Controls

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