Trastuzumab Antibody | AbD18018_hIgG1

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Trastuzumab Antibody | AbD18018_hIgG1 gallery image 1

Human anti Trastuzumab (Herceptin® specificity ELISA (clone AbD18018 in IgG1 format)
A microtiter plate was coated over night using various antigens (A: 20% human serum, B: IgG1/kappa from human myeloma plasma, C: Rituximab, D: Bevacizumab, E: Ustekinumab, F: Infliximab, G: Adalimumab, H: Trastuzumab as coating components. After washing and bolcking with PBST+5% BSA, detection was perfomed using Human anti Trastuzumab:HRP in its IgG1 format (clone AbD18018_hIgG1, HCA177) as the detection reagent

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Human anti trastuzumab (Herceptin®) bridging ELISA for Anti-Drug Antibody (ADA) assay development (clone AbD18018 in IgG1 format)
A microtitre plate was coated over night with trastuzumab at a concentration of 0.5 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human anti Trastuzumab, clone AbD18018_hIgG1 (HCA177) and HRP conjugated Trastuzumb as the detection reagent

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Trastuzumab (Herceptin®) bridging ELISA for Pharmacokinetic (PK) assay development (clone AbD18018 in IgG1 format)

A microtiter plate was coated over night with Human anti trastuzumab, clone AbD16712 (HCA166) (black) or clone AbD18141 (HCA169) (red) at a concentration of 1 μg/ml. After washing and blocking with PBST +5% BSA in, 10 % human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed by HRP-conjugated Human anti trastuzumab, AbD16712-hIgG1 (HCA177P) at a concentration of 2 μg/ml in HiSpec assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate

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  • Human anti Trastuzumab:HRP
  • Human anti Trastuzumab
(Rated 5.0 out of 5 based on 1 customer reviews)
    Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical method development. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18018_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA177PEdatasheet pdfdatasheet pdf0.1 mg
      HCA177P
      HCA177Edatasheet pdfdatasheet pdf0.1 mg
      HCA177
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti trastuzumab, clone AbD18018_hIgG1, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.

        Clone AbD18018_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug in preclinical research and clinical trials using patient sera. This antibody, in full immunoglobulin format and conjugated to HRP, is recommended as the detection antibody, paired with antibody clone AbD16712 (HCA166) or clone AbD18141 (HCA169), both monovalent Fab format, as the capture antibody.

        Trastuzumab, also known as Herceptin®, is a drug used in the treatment of HER2 postive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

        View a summary of all anti-trastuzumab antibodies
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Trastuzumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.02 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
      • Approx. Protein Concentrations
        IgG concentration 0.1mg/ml
        IgG concentration 0.5mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin® is a registered trademark of Genentech, Inc.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin® is a registered trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used as a detection reagent in a sandwich ELISA together with HCA169 as the capture reagent.

        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Trastuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Trastuzumab Antibody : Purified AbD18018_hIgG1 E 0.1 mg
      Trastuzumab Antibody : HRP AbD18018_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            MCA647P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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                Fantastic Antibody
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                Submitted: 6 Jul 2016
                Always very happy with the material we purchase from Bio-Rad & AbD

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