Trastuzumab Antibody | AbD16714

Trastuzumab Antibody | AbD16714 gallery image 1

Herceptin® bridging ELISA using Human anti Herceptin® (HCA169) as the coating antibody; Herceptin® as the antigen and Human anti Herceptin® (HCA167) conjugated to HRP as the detection antibody

Trastuzumab Antibody | AbD16714 gallery image 2

Herceptin® ELISA using Human anti Herceptin® (HCA167) as the coating antibody; Herceptin® as the antigen and Mouse anti Human IgG (Fc):HRP (MCA647P) as the detection antibody

Trastuzumab Antibody | AbD16714 gallery image 3

HCA167 specificity ELISA using various antigens (A: BSA, B: CD33, C: GST, D: 20% human serum, E: IgG1/kappa from human myeloma plasma, F: Alemtuzumab, G: Bevacizumab, H: Herceptin®) as coating components; Human anti Herceptin® (HCA167) as the primary antibody and Mouse anti Strep-tag:HRP (MCA2489P) as the detection antibody

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  • Human anti Trastuzumab
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    Anti-trastuzumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated for direct ELISA and in a PK bridging ELISA format.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA167Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

        Clone 16714 is an anti-idiotypic antibody that specifically recognises the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.

        Clone 16714 has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette (EQU003) for this purpose.

        Additional Trastuzumab (Herceptin) specific anti-idiotypic antibodies are available upon request.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
      • Reconstitution
      • Preparation
        StrepTactin affinity chromatography
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
      • Purity
      • Affinity
        The monovalent intrinsic affinity of HCA167 was measured as 2.5nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin® is a registered trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Trastuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Trastuzumab Antibody : Purified AbD16714 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
        Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
        Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        Mouse anti Strep-Tag ImmoMCA24880.1 mgE

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-V5Sx2 Negative ControlHCA1250.1 mgC, E, F, P, WB

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC*, E
            Human anti TrastuzumabHCA1660.1 mgE
            Human anti TrastuzumabHCA1680.1 mgE
            Human anti TrastuzumabHCA1690.1 mgE
            Human anti TrastuzumabHCA1760.1 mgE
            Human anti TrastuzumabHCA1770.1 mgE
            Hispec Assay DiluentBUF049A50 mlIY

            Recommended Positive Controls

              Histology Controls

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