Trastuzumab Antibody | 16714

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Herceptin® bridging ELISA using Human anti Herceptin® (HCA169) as the coating antibody; Herceptin® as the antigen and Human anti Herceptin® (HCA167) conjugated to HRP as the detection antibody

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Herceptin® ELISA using Human anti Herceptin® (HCA167) as the coating antibody; Herceptin® as the antigen and Mouse anti Human IgG (Fc):HRP (MCA647P) as the detection antibody

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HCA167 specificity ELISA using various antigens (A: BSA, B: CD33, C: GST, D: 20% human serum, E: IgG1/kappa from human myeloma plasma, F: Alemtuzumab, G: Bevacizumab, H: Herceptin®) as coating components; Human anti Herceptin® (HCA167) as the primary antibody and Mouse anti Strep-tag:HRP (MCA2489P) as the detection antibody

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  • Human anti Trastuzumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-trastuzumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated for direct ELISA and in a PK bridging ELISA format.
    • Product Type
      Monoclonal Antibody
    • Clone
      16714
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA167Edatasheet pdfdatasheet pdf0.1 mg
      HCA167
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

        Clone 16714 is an anti-idiotypic antibody that specifically recognises the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.

        Clone 16714 has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette (EQU003) for this purpose.

        Additional Trastuzumab (Herceptin) specific anti-idiotypic antibodies are available upon request.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
      • Reconstitution
      • Preparation
        StrepTactin affinity chromatography
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Herceptin
      • Purity
      • Affinity
        The monovalent intrinsic affinity of HCA167 was measured as 2.5nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin is a registered trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Trastuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Trastuzumab Antibody : Purified 16714 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
        MCA1360A
        Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
        MCA1360B
        Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
        MCA1360P
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        MCA2489P
        Mouse anti Strep-Tag ImmoMCA24880.1 mgE
        MCA2488

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-V5Sx2 Negative ControlHCA1250.1 mgC, E, F, P, WB
          HCA125

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            MCA647P
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1680.1 mgE
            HCA168
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Human anti TrastuzumabHCA1760.1 mgE
            HCA176
            Human anti TrastuzumabHCA1770.1 mgE
            HCA177
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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