TMB Core Prestained

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  • TMB Core Prestained
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF057BEdatasheet pdfdatasheet pdf500 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Intended Use
      BUF057B is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP). TMB CORE PRESTAINED is stained with a red indicator to eliminate pipetting errors. The red indicator does not interfere with the sensitivity or the stability of the substrate. It does not absorb light at the wavelengths used for the substrate and will disappear after addition of the stop solution.

      TMB CORE PRESTAINED contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution.

      BUF057B produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
    • Product Form
      Ready to use TMB solution - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product is photosensitive and should be protected from light.

      Avoid exposure to heat and contamination with metal ions or peroxidase.

      Store in bottles made of High Density Polyethylene (HDPE).
    • Shelf Life
      24 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Pour the desired amount of BUF057B into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well.

      2. Add 0.1ml of BUF057B per microtiter well.

      3. Allow development of BUF057B. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil.

      4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

      5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF057B is not recommended).

    Additional TMB Core Prestained Formats

    Formats Applications Sizes available
    TMB Core Prestained : Ready To Use E 500 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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