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TMB Core+

Product Code Applications Pack Size List Price Your Price Qty
BUF062B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml loader
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loader
BUF062C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml loader
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loader
BUF062A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml loader
List Price Your Price
loader

Intended Use
BUF062B: BUF062B is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).

BUF062B contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development.

BUF062B produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
BUF062C: BUF062C is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).

BUF062C contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development.

BUF062C produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
BUF062A: BUF062A is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).

BUF062A contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development.

BUF062A produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Product Form
Ready to use TMB solution - liquid
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product is photosensitive and should be protected from light.

Avoid exposure to heat and contamination with metal ions or peroxidase.

Store in bottles made of High Density Polyethylene (HDPE).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. It is recommended that 100ul of BUF062B TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.

2. Add 100ul substrate solution per microtiter well.

3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF062B is not recommended).
1. It is recommended that 100ul of BUF062C TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.

2. Add 100ul substrate solution per microtiter well.

3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF062C is not recommended).
1. It is recommended that 100ul of BUF062A TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.

2. Add 100ul substrate solution per microtiter well.

3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF062A is not recommended).

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+
TMB Core+ BUF062C E 1000 ml
List Price Your Price
Description TMB Core+
TMB Core+ BUF062B E 500 ml
List Price Your Price
Description TMB Core+

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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