This assay must only be performed by an authorised radioisotope user and safety procedures for 3H-thymidine must be followed in a designated radioactive area. This assay measures IL-2 and Phytohemagglutinin (PHA) induced proliferation of primary chicken thymocytes by incorporation of 3H-thymidine. To ensure proliferation is due to IL-2 and not other cytokines e.g IL-1ß, pre-incubation of samples with a neutralizing antibody is essential.
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