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Texas Red® Conjugation Kit

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  • LYNX Rapid Texas Red® Antibody Conjugation Kit
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Conjugation Kit
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    LNK181TRCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 200µg Antibody
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • LYNX Rapid Texas Red® Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Texas Red® label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in the coupling of Texas Red® to the antibody.

      The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      3 Vials of LYNX lyophilized Texas Red® mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
    • Preparing The Antibody
      The following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-200ug of antibody be used in each labelling reaction. For optimal results the antibody volume should be around 40-100ul, at a concentration range of 1-4mg/ml.
    • Test Principle
    • Buffer Solution
    • Storage
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
    • Shelf Life
      12 months after despatch
    • Acknowledgements
    • Licensed Use
      Bio-Rad LYNX Rapid Conjugation kits® are offered for research purposes alone, and are not intended for human, therapeutic or diagnostic use. The purchase of this conjugation kit conveys to the buyer (whether the buyer is anot-for-profit, academic or for-profit entity) the non-transferableright to use the amount of product purchased andthe components of the product for in-house research. Thebuyer shall not sell or otherwise transfer this product, itscomponents, or materials prepared therefrom to any thirdparty. The buyer shall not use this product or its componentsfor commercial purposes. For the avoidance of doubt,‘commercial purposes’ means any activity by a party forconsideration and includes, without limitation, use of theproduct or its components (i) in the manufacturing ofconjugated materials (e.g. labeled antibodies), (ii) to providea service, information or data, (iii) for therapeutic, diagnosticor prophylactic purposes, or (iv) for repackaging/resale,whether or not such product or its components are resold foruse in research. The use of this product by the buyerconstitutes agreement with the terms of this limited use labellicense for LYNX products.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    Additional Texas Red® Conjugation Kit Formats

    Formats Applications Sizes available
    Texas Red® Conjugation Kit : Kit CJ 3 Conjugations For 200µg Antibody
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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