100% secure
Email this product page
Print this page

Retinoblastoma Gene Protein (pThr356) Antibody | 7F10

Retinoblastoma Gene Protein (pThr356) Antibody | 7F10 gallery image 1

Western blot of recombinant RB. Human RB protein was purified from E. coli and either Mock phosphorylated (MOCK) or phosphorylated in vitro using recombinant Cyclin D1/ CDK4 complex (D1/K4) or Cyclin E/ CDK2 complex (E/K2). The blot was probed with MCA2104 recognizing Rb phosphorylated in residue T356. The blot was stripped and subsequently counter-probed with pan Rb antiserum

  • Retinoblastoma Gene Protein (pThr356) Antibody | 7F10 thumbnail image 1
  • Rat anti Retinoblastoma Gene Protein (pThr356)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2104C, WB*datasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti phosphorylated human retinoblastoma associated protein, clone 7F10 recognises both human and mouse Retinablastoma protein protein only when it has been phosphorylated at the T356 residue.

      The antibody does not recognise non-phosphorylated Rb or Rb phosphorylated at other sites.

      Phosphorylation of the Rb protein at T356 is dependent upon cyclin-dependent kinase 4.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting(1)1/1001/500
      For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Retinoblastoma Gene Protein (pThr356) Antibody Formats

    Formats Clone Applications Sizes available
    Retinoblastoma Gene Protein (pThr356) Antibody : Purified 7F10 C, WB* 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Retinoblastoma Gene Protein (pThr356) Antibody | 7F10 thumbnail image 1


              Write your review

              You may also be interested in...