Retinoblastoma Gene Protein (pThr356) Antibody | 7F10

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Retinoblastoma Gene Protein (pThr356) Antibody | 7F10 gallery image 1

Western blot of recombinant RB. Human RB protein was purified from E. coli and either Mock phosphorylated (MOCK) or phosphorylated in vitro using recombinant Cyclin D1/ CDK4 complex (D1/K4) or Cyclin E/ CDK2 complex (E/K2). The blot was probed with MCA2104 recognizing Rb phosphorylated in residue T356. The blot was stripped and subsequently counter-probed with pan Rb antiserum

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  • Rat anti Retinoblastoma Gene Protein (pThr356)
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  • Product Type
    Monoclonal Antibody
  • Clone
    7F10
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2104C, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA2104
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti retinoblastoma gene protein (pThr356) antibody, clone 7F10 recognizes retinoblastoma gene protein, also known as retinoblastoma-associated protein, p105-Rb, pp110 and Rb, when phosphorylated at threonine 356.

      Phosphorylation of the Rb protein at threonine 356 is dependent upon cyclin-dependent kinase 4.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting(1)1/1001/500
      (1)
      For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Retinoblastoma Gene Protein (pThr356) Antibody Formats

    Formats Clone Applications Sizes available
    Retinoblastoma Gene Protein (pThr356) Antibody : Purified 7F10 C, WB* 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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