hnRNP K antibody | F45 P9 C7
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Mouse anti hnRNP K
- Product Type
- Monoclonal Antibody
- Clone
- F45 P9 C7
- Isotype
- IgG1
- Specificity
- hnRNP K
Antibody Quality and Performance Guarantee
| Mouse anti hnRNP K antibody, clone F45 P9 C7 recognizes hnRNP K (heterogeneous ribonucleotide protein K), a 464 amino acid acidic protein found in the nucleus, cytoplasm and mitochondria, involved in transcription, mRNA shuttling and the translation and editing of RNA. Over-expression of hnRNP K has been demonstrated in cancerous tissue including that of the colon, breast, liver and lungs, and its involvement in cell migration has been implicated in tumour metastasis. hnRNP K increases the transcriptional activity of the c-myc and c-src oncogenes (Wang et al. 2020). |
- Target Species
- Synthetic Peptide
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Rabbit Mouse Chicken Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Ovalbumin-conjugated synthetic peptide SVKQYSGKFF
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
1/200 | 1/500 |
| Western Blotting | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Colon carcinoma
- Western Blotting
- MCA2622GA detects a band of approximately 51kDa in Calu6 and HT29 cell lysates.
References for hnRNP K antibody
-
Carpenter, B. et al. (2006) Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer.
Br J Cancer. 95 (7): 921-7.
Further Reading
-
Bomsztyk, K. et al. (1997) Diverse molecular interactions of the hnRNP K protein.
FEBS Lett. 403 (2): 113-5.
- Synonyms
- Heterogenous Nuclear Ribonucleoprotein K
- RRID
- AB_1102608
- UniProt
- P61978
- Entrez Gene
- HNRNPK
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003677 DNA binding
- GO:0003723 RNA binding
- GO:0007165 signal transduction
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005681 spliceosomal complex
- GO:0030530 heterogeneous nuclear ribonucleoprotein complex
- View More GO Terms
- GO:0044419 interspecies interaction between organisms
MCA2622GA
153994
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