Histone H4 Ac8 Antibody

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Histone H4 Ac8 Antibody gallery image 1

Published customer image:
Rabbit anti Histone H4 (Ac8) antibody (AHP415) used for the evaluation of acetylated histone expression in yeast cell lysates by western blotting.
Image caption:
Eaf1p Was a Subunit of the NuA4 HAT (A) Eaf1-HA associated with NuA4 subunits. Immunoblots of analytical-scale TAP purifications are shown. The captured TAP-tagged protein is indicated above the gels, and the protein that was tested for association is indicated at the right. (B) Strains lacking EAF1 have defects in histone H4 acetylation. Whole cell extracts from mutant strains indicated on the top were tested for global histone acetylation using antibodies directed against different forms of acetylated H4 and H2A as indicated on the right.

From: Kobor MS, Venkatasubrahmanyam S, Meneghini MD, Gin JW, Jennings JL, Link AJ, et al. (2004)
A Protein Complex Containing the Conserved Swi2/Snf2-Related ATPase Swr1p Deposits Histone Variant H2A.Z into Euchromatin.
PLoS Biol 2(5): e131.

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Histone H4 Ac8 Antibody gallery image 2

Published customer image:
Rabbit anti Histone H4 AcK8 antibody (AHP415) used for the assessment of histone H4 acetylation at lysine 8 in a rat model of colitis by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (A), 8 (K8) (B), 12 (K12) (C) and 16 (K16) (D) in a Sham (control) and trinitrobenzene sulfonic acid (TNBS) rat model of colitis. Results were obtained by Western blotting. In order to evaluate changes in density from different western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean &pluxmn; SEM). (*p <0.05 vs Sham proximal or Sham distal respectively).

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan27;8(1):1.

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Histone H4 Ac8 Antibody gallery image 3

Published customer image:
Rabbit anti Histone H4 AcK8 antibody (AHP415) used for the assessment of histone H4 acetylation at lysine 8 in a Lewis and Sprague Dawley rat model of DSS treatment by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5), 8 (K8), 12 (K12) and 16 (K16) in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS). A: Representative bands of H4K5, K8, K12 and K16 acetylation. Lanes for Lewis rats represent: non-DSS treated (control) and DSS-treated. Likewise representative bands are illustrated for the Sprague-Dawley rats. Graphical representation of Western blotting data. H4 acetylation of K5 (B), K8 (C), K12 (D) and K16 (E). Columns represent the mean ± SEM (bar) of three independent experiments.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan27;8(1):1.

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Histone H4 Ac8 Antibody gallery image 4

Published customer image:
Rabbit anti Histone H4 AcK8 antibody (AHP415) used for the assessment of histone H4 acetylation at lysine 8 in Chron~s disease biopsy material lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) and H4 lysine residues in Crohn's disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 μg of protein) (*p <0.05 vs control). Pan acetylation on H4 in Crohn's disease (A). Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer's patches of Crohn's disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p <0.05 vs control, #p <0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan27;8(1):1.

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP415CH, E, IF, IP, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP415
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Histone 4(Ac8) antibody recognizes histone H4 only when acetylated at lysine 8.
    • Intended Use
    • Target Species
      Synthetic Peptide
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Drosophilayes
      Yeastyes
      Mammalsyes
      Amphibiayes
      Plantsyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Ovalbumin-conjugated peptide: NSGRGKGGAcKGLGKYCc.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      nucleoplasm
      protein binding
      phosphatidylinositol-mediated signaling
      nucleosome
      CenH3-containing nucleosome assembly at centromere
      DNA binding
      negative regulation of megakaryocyte differentiation
      telomere maintenance
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Chromatin Immunoprecipitation
      ELISA1/800
      Immunofluorescence1/500
      Immunoprecipitation
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Histone H4 Ac8 Antibody Formats

    Formats Applications Sizes available
    Histone H4 Ac8 Antibody : Serum CH, E, IF, IP, WB 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              References

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