Browsing Modes for Business Users

Histidine Tag antibody

Goat anti Histidine Tag

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histidine Tag

Product Code Applications Pack Size List Price Your Price Qty
AHP1656
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E * WB 0.1 mg loader
List Price Your Price
loader

Goat anti-Histidine tag antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins.

Target Species
Synthetic Peptide
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Histidine Tag was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 1/1000 1/30000
Western Blotting
  1. 1We recommend a dilution range of 1/1000 to 1/30000 for use in indirect ELISA. When coating the antibody directly on to a plate, Bio-Rad recommend a dilution range to 1/100 to 1/500.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

RRID
AB_1658087

AHP1656

150794 155031 159613 159787

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Please note