Histidine Tag Antibody

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  • Goat anti Histidine Tag
  • Goat anti Histidine Tag:FITC
  • Goat anti Histidine Tag:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1656E *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1656
    AHP1656FIFdatasheet pdfdatasheet pdf0.1 mg
    AHP1656F
    AHP1656BE, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1656B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti-Histidine tag antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins.
    • Intended Use
    • Target Species
      Synthetic Peptide
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to Histidine Tag was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      ELISA(1)1/10001/30000
      (1)
      We recommend a dilution range of 1/1000 to 1/30000 for use in indirect ELISA. When coating the antibody directly on to a plate, Bio-Rad recommend a dilution range to 1/100 to 1/500.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1:100001:100000
      Western Blotting1:100001:100000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Histidine Tag Antibody Formats

    Formats Applications Sizes available
    Histidine Tag Antibody : Purified E *, WB 0.1 mg
    Histidine Tag Antibody : Biotin E, WB 0.1 mg
    Histidine Tag Antibody : FITC IF 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Proteus IMAC MIDI Purification KitPUR0218 UnitsPP
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          Proteus IMAC MIDI Purification Spin Column Pack + BuffersPUR0198 UnitsPP
          PUR019
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          PUR021
          Proteus IMAC MIDI Purification Spin Column Pack + BuffersPUR0198 UnitsPP
          PUR019
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Proteus IMAC MIDI Purification KitPUR0218 UnitsPP
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          Proteus IMAC MIDI Purification Spin Column Pack + BuffersPUR0198 UnitsPP
          PUR019

          Recommended Positive Controls

            Histology Controls

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