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Histidine Tag antibody | AD1.1.10

Mouse anti Histidine Tag

Product Type
Monoclonal Antibody
Clone
AD1.1.10
Isotype
IgG1
Specificity
Histidine Tag

Product Code Applications Pack Size List Price Your Price Qty
MCA1396GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IP WB 0.1 mg loader
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loader
MCA1396G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IP WB 2 mg loader
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loader
MCA1396
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IP WB 1 mg loader
List Price Your Price
loader

Mouse anti Histidine tag antibody, clone AD1.1.10 recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.

In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Target Species
Synthetic Peptide
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
PAX6 transcription factor linked to histidine tag.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
MCA1396GA, MCA1396: His-tag is a trademark of EMD Biosciences.
MCA1396G: His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoprecipitation
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Histidine Tag antibody

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ELISA

Flow Cytometry

Immunoprecipitation

Western Blotting

RRID
AB_322084

MCA1396GA

154982 163612 164567 1807

MCA1396G

154295 162000 163640 164570

MCA1396

0215 154116 164571

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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