Substance P antibody | NC1/34
Rat anti substance P antibody, clone NC1/34 (8450-0505) used for the detection of substance P expressing cells in human tendon tissue biopsies by immunofluorescence.
Image caption:
Double stainings for PARs and substance P. A-D shows PARs [1]-[4] and E-H shows corresponding SP-staining; PAR-1 (A) is co-localised with SP-positive nerve fibres (E) (arrows). The same was seen for PAR-2 (nerve fascicle in B&F), PAR-3 (nerve fascicle in C&G) and PAR-4 (D&H, arrows show perivascular nerve fibres).
From: Christensen, J. Alfredson, H. and Andersson, G. "Protease-activated receptors in the Achilles tendon–a potential explanation for the excessive pain signalling in tendinopathy".
Mol Pain. 2015 Mar 17;11:13.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat antiSubstance P antibody, clone NC1/34 (8450-0505) used for the identification of substance P immunoreactive neurons in porcine dorsal root ganglia by immunofluorescence.
Image caption:
Representative images of substance P-positive (SP+) dorsal root ganglia (DRG)-urinary bladder-projecting neurons (UBPN) in control pigs. All images were taken separately from blue (a,d,g,j,m,p), green (b,e,h,k,n,r) and red (c,f,i,l,o,s) fluorescent channels; a–c One fast blue-positive (FB+) neuron (a, blue, arrow), which simultaneously contains SP (b, green, arrow) and pituitary adenylate cyclase activating peptide-PACAP (c, red, arrow). d–f One FB+ neuron (d, blue, arrow), which is simultaneously SP+ (e, green, arrow) and calcitonin gene-related peptide-positive (CGRP+, f, red, arrow). g–i Two FB+ neurons (g, blue, 1 short arrow, 1 long arrow), which are simultaneously SP+ (h, green, 1 short arrow) or SP-negative (h, green, 1 long arrow) and galanin-positive (GAL+, i, red, 1 short arrow) or GAL-negative (GAL-, i, red, 1 red arrow). j–l One FB+ neuron (j, blue, arrow), which simultaneously contains neuronal nitric oxide synthase-nNOS (k, green, arrow) and SP (l, red, arrow). m–o One FB+ neuron (m, blue, arrow), which is simultaneously calbindin-positive (CB+, n, green, arrow) and SP+ (o, red, arrow). p-s One FB+ neuron (p, blue, arrow), which simultaneously contains SP (r, green, arrow) and somatostatin-SOM (s, red, arrow). Bars 50 μm (a–s).
From: Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W. Botulinum Toxin Type A Induces Changes in the Chemical Coding of Substance P-ImmunoreactiveDorsal Root Ganglia Sensory Neurons Supplying the Porcine Urinary Bladder.
Toxins (Basel). 2015 Nov 16;7(11):4797-816.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat antiSubstance P antibody, clone NC1/34 (8450-0505) used for the identification of substance P immunoreactive neurons in porcine dorsal root ganglia by immunofluorescence.
Image caption:
Representative images of substance P-positive (SP+) dorsal root ganglia (DRG)-urinary bladder-projecting neurons (UBPN) in BTX-treated animals. All images were taken separately from blue (a,d,g,j,m,p), green (b,e,h,k,n,r) and red (c,f,i,l,o,s) fluorescent channels; a–c one fast blue-positive (FB+) neuron (a, blue, arrow), which simultaneously contains SP (b, green, arrow) and pituitary adenylate cyclase activating peptide-PACAP (c, red, arrow). d–f One FB+ neuron (d, blue, arrow), which is simultaneously SP+ (e, green, arrow) and calcitonin gene-related peptide-positive (CGRP+, f, red, arrow). g–i One FB+ neuron (g, blue, arrow), which is simultaneously SP+ (h, green, arrow) and galanin-positive (GAL+, i, red, arrow). j–l One FB+ neuron (j, blue, arrow), which simultaneously contains neuronal nitric oxide synthase-nNOS (k, green, arrow) and SP (l, red, arrow). m–o One FB+ neuron (m, blue, arrow), which is simultaneously SP+ (n, green, arrow) and calbindin-negative (CB-, o, red, arrow). p-s One FB+ neuron (p, blue, arrow), which simultaneously contains SP (r, green, arrow) and somatostatin-SOM (s, red, arrow). Bars 50 μm (a–s).
From: Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W. Botulinum Toxin Type A Induces Changes in the Chemical Coding of Substance P-ImmunoreactiveDorsal Root Ganglia Sensory Neurons Supplying the Porcine Urinary Bladder.
Toxins (Basel). 2015 Nov 16;7(11):4797-4816.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used for the illustration of substance P expressing cells in porcine medulla oblongata by immunofluorescence.
Image caption:
a, FB-labeled neurons (arrows) in the porcine control DMX. b, GAL-IR fibers (arrowheads) in the DMX. c, GAL-IR fibers (arrowheads) in close proximity of the FB-labeled somata (arrows) in the DMX. d, FB-labeled neurons (arrows) in the porcine control DMX. e, SP-IR fibers (arrowheads) in the DMX. f, SP-IR fibers (arrowheads) surrounded the FB-labeled somata (arrows) enabling direct contact. g, FB-labeled neurons (arrows) in the porcine control DMX. h, LENK-IR fibers (arrowheads) in the DMX. i, LENK-IR fibers (arrowheads) formed basket-like structures encircling FB-labeled neurons (arrows) in the DMX. j, FB-labeled neuron (arrow) in the porcine control DMX. k, CART-IR fibers (arrowheads) in the DMX. l, CART-IR fibers (arrowheads) in close proximity of the FB-positive perikaryon (arrow) in the DMX. Photographs of Fig. c, f, i, l were prepared by superimposition of single image
From: Gańko M, & Całka J.
Prolonged acetylsalicylic-acid-supplementation-induced gastritis affects the chemical coding of the stomach innervating vagal efferent neurons in the porcine dorsal motor vagal nucleus (DMX).
J Mol Neurosci. 2014;54(2):188-98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Substance P anitbody, clone NC1/34 (8450-0505) used for the evaluation of substance P expression on rabbit muscle nerve fasciles by immunofluorescence.
Image captionTachykinin-like immunoreactivity in nerve fascicles. Nerve fascicles and parts of nerve fascicles (N) of sections from gastrocnemius (A-C, E, F) and soleus (D) muscle specimens are shown. The sections were processed for demonstration of tachykinin-like immunoreaction (A-E). In (F) the reaction pattern after preabsorption with SP peptide is shown (the region in F corresponds to the region in E). The antibodies applied were sc-14104 (A-C, E) and 8450–0505 (D). The specimens were from the control group (A, B, E, F) and from the 6w group, non-exercised side, focal myositis area (C, D). There are immunoreactivities in nerve fascicles in the form of punctuate reactions (arrows A, C-E). These are grouped, as especially seen in (C), but also to some extent in (D). There are no reactions in the nerve fascicle in (B), which is built up of myelinated nerve fibers, and in the control section in (F). MF refers to muscle fibers. Magnification x200 (B, C), x315 (A, D, E, F).
From: Song Y, Stål PS, Yu JG, Forsgren S.
Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
BMC Musculoskelet Disord. 2013 Apr 12;14:134.
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Rat anti Substance P anitbody, clone NC1/34 (8450-0505) used for the evaluation of substance P expression on rabbit muscle nerve fasciles by immunofluorescence.
Image captionA,B. Tachykinin-like immunoreactivity in cells in inflammatory infiltrate of myositis area. Sections of a specimen from a soleus muscle specimen of the 6w group, non-exercised side. In (A), the section had been immunostained with 8450–0505, and in (B), the section was immunostained with 8450–0505 after preabsorption with tachykinin (SP) (larger area is covered in B than in A). Immunoreactivity is seen in cells in (A), but no reaction is visible in (B). Arrows point at cells of inflammatory infiltrate. The immunoreactions show partly a granular appearance (A). Magnification x315. c,d. Immunostaining with polyclonal and monoclonal tachykinin antibodies. A part of a focal myositis area of a section of a gastrocnemius muscle specimen from the 1w group, exercised side, double-stained for tachykinin using the sc-14104 (C) and 8450–0505 (D) antibodies, shown at low magnification. Note that the cells of an inflammatory infiltrate are immunolabelled in both (C) and (D) (arrows), whereas the wall of a small vessel is only immunostained in (C) (asterisks). Magnification x315.
From: Song Y, Stål PS, Yu JG, Forsgren S.
Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
BMC Musculoskelet Disord. 2013 Apr 12;14:134.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Substance P antibody, clone NC1/34 (8450-0505) used for the demonstration of neurons in porcine descending colon by immunofluorescence.
Image caption:
Representative images of co-localization of calcitonin gene-related peptide (CGRP) with other neuronal active substances in the neurons of myenteric plexus (I,II,III) and nerve fibers scattered in the circular muscle layer (IV) of the porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration. I—co-localization of CGRP with substance P (SP); II—co-localization of CGRP with galanin (GAL). Images are composites of merged images taken separately from green (CGRP) and red (other substances studied) fluorescent channels. Nervous structures, where CGRP co-localizes with other substances, are indicated by arrows.
From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Substance P antibody, clone NC1/34 (8450-0505) used for the demonstration of neurons in porcine descending colon by immunofluorescence.
Image caption:
Representative images of co-localization of calcitonin gene-related peptide (CGRP) with other neuronal active substances in the neurons of the outer submucous plexus in the wall of porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration. I—co-localization of CGRP with substance P (SP); II—co-localization of CGRP with galanin (GAL); III—co-localization of CGRP with cocaine- and amphetamine- regulated transcript (CART) peptide. Images are composites of merged images taken separately from green (CGRP) and red (other substances studied) fluorescent channels. Neurons, where CGRP co-localizes with other substances are indicated by arrows.
From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Substance P antibody, clone NC1/34 (8450-0505) used for the demonstration of neurons in porcine descending colon by immunofluorescence.
Image caption:
Representative images of co-localization of calcitonin gene related peptide (CGRP) with other neuronal active substances in the neurons of inner submucous plexus (I,II,III) and nerve fibers scattered in submucous/mucous layer (IV) of the porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration. I—co-localization of CGRP with substance P (SP); II—co-localization of CGRP with galanin (GAL). Images are composites of merged images taken separately from green (CGRP) and red (other substances studied) fluorescent channels. Nervous structures, where CGRP co-localizes with other substances are indicated by arrows.
From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to demonstrate Substance P expressing cells in ovine urethral submucosal tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Illustration of an SP-IR-positive stain (arrow) in the urethral submucosa of a lamb. The stains are located within the nerve fibers of ganglia (diamond) or close to blood vessels (stars).
From: Sickinger M, Wenisch S, Wehrend A.
Neuropeptides in the urinary tract of male sheep lambs.
Res Vet Sci. 2020 Oct 18:S0034-5288(20)31051-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to demonstrate substance P expression by neurons in porcine submucosal plexus with immunofluorescence.
Image caption:
Outer submucous plexus in control animals (a) and after BPA treatment in low (b) and high (c) doses, immunostained for protein gene product 9.5 (PGP 9.5, a pan-neuronal marker) and other neuronal factors including substance P (SP) and vasoactive intestinal polypeptide (VIP). Neurons showing co-localization of PGP 9.5 and other neuronal factors are indicated with arrows.
From: Szymanska K, Makowska K, Gonkowski S.
The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018 Mar 20;19(3):917.
doi: 10.3390/ijms19030917.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to demonstrate substance P expression by neurons in porcine submucosal plexus with immunofluorescence.
Image caption:
Inner submucous plexus in control animals (a) and after BPA treatment in low (b) and high (c) doses, immunostained for protein gene product 9.5 (PGP 9.5, a pan-neuronal marker) and other neuronal factors including substance P (SP) and vasoactive intestinal polypeptide (VIP). Neurons showing co-localization of PGP 9.5 and other neuronal factors are indicated with arrows.
From: Szymanska K, Makowska K, Gonkowski S.
The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018 Mar 20;19(3):917.
doi: 10.3390/ijms19030917.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label substance P expressing cells from porcine nodose ganglia by immunofluorescence.
Image caption:
Fluorescence photographs showing perikarya (arrows) immunoreactive to the following: (a) FB (A), SP (B), and CGRP (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the control group. (b) FB (A), SP (B), and CGRP (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the ASA group.
From: Rytel L, Całka J.
Aspirin Administration Affects Neurochemical Characterization of Substance P-Like Immunoreactive (SP-LI) Nodose Ganglia Neurons Supplying the Porcine Stomach.
Biomed Res Int. 2020 Jun 12;2020:1049179.
doi: 10.1155/2020/1049179..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label substance P expressing cells from porcine nodose ganglia by immunofluorescence.
Image caption:
Fluorescence photographs showing perikarya (arrows) immunoreactive to the following: (c) FB (A), SP (B), and nNOS (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the control group. (d) FB (A), SP (B), and nNOS (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the ASA group.
From: Rytel L, Całka J.
Aspirin Administration Affects Neurochemical Characterization of Substance P-Like Immunoreactive (SP-LI) Nodose Ganglia Neurons Supplying the Porcine Stomach.
Biomed Res Int. 2020 Jun 12;2020:1049179.
doi: 10.1155/2020/1049179..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label substance P expressing cells from porcine nodose ganglia by immunofluorescence.
Image caption:
Fluorescence photographs showing perikarya (arrows) immunoreactive to the following: (a) FB (A), SP (B), and VIP (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the control group. (b) FB (A), SP (B), and VIP (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the ASA group.
From: Rytel L, Całka J.
Aspirin Administration Affects Neurochemical Characterization of Substance P-Like Immunoreactive (SP-LI) Nodose Ganglia Neurons Supplying the Porcine Stomach.
Biomed Res Int. 2020 Jun 12;2020:1049179.
doi: 10.1155/2020/1049179..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label substance P expressing cells from porcine nodose ganglia by immunofluorescence.
Image caption:
Fluorescence photographs showing perikarya (arrows) immunoreactive to the following: (c) FB (A), SP (B), and GAL (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the control group. (d) FB (A), SP (B), and GAL (C); (D) shows double labeling FB/SP/CGRP of the nodose ganglion neurons in the ASA group.
From: Rytel L, Całka J.
Aspirin Administration Affects Neurochemical Characterization of Substance P-Like Immunoreactive (SP-LI) Nodose Ganglia Neurons Supplying the Porcine Stomach.
Biomed Res Int. 2020 Jun 12;2020:1049179.
doi: 10.1155/2020/1049179..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti substance P antibody, clone NC1/34 (8450-0505) used to label porcine myenteric neurons in conjunction with PGP9.5 using Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) by immunofluorescence.
Image caption:
Immunofluorescence findings of ENS neurons in the myenteric plexuses. Representative images of duodenum myenteric neurons immunopositive to SP, in physiological state (A), after low (B) and high (C) doses of acrylamide supplementation. (A-C) - neurons immunopositive to protein gene-product 9.5 (PGP9.5) - used as a panneuronal marker and SP; All photographs have been made by overlapping of green and red fluorescent channels (green for PGP 9.5 and red for SP). Neurons immunopositive to particular substance studied are indicated with arrows.
From: Palus K, Całka J.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 2019 Dec 18;21(1):15.
doi: 10.3390/ijms21010015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti substance P antibody, clone NC1/34 (8450-0505) used to label porcine myenteric neurons in conjunction with PGP9.5 using Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) by immunofluorescence.
Image caption:
Immunofluorescence findings of the ENS neurons in the outer submucous plexuses. Representative images of duodenum other submucous neurons immunopositive to SP in physiological state (A), after low (B) and high (C) doses of acrylamide supplementation. (A–C)—outer submucous neurons immunopositive to protein gene-product 9.5 (PGP9.5)—used as a panneuronal marker and SP; All photographs have been made by overlapping of green and red fluorescent channels (green for PGP 9.5 and red for SP). Neurons immunopositive to the particular substance studied are indicated with arrows.
From: Palus K, Całka J.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 2019 Dec 18;21(1):15.
doi: 10.3390/ijms21010015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti substance P antibody, clone NC1/34 (8450-0505) used to label porcine myenteric neurons in conjunction with PGP9.5 using Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) by immunofluorescence.
Image caption:
Immunofluorescence findings of the ENS neurons in the inner submucous plexuses. Representative images of duodenum inner submucous neurons immunopositive to SP, CGRP, GAL, nNOS, and VACHT in physiological state (A,D,G,J,M), after low (B,E,H,K,N) and high (C,F,I,L,O) doses of acrylamide supplementation. (A–C)—inner submucous neurons immunopositive to protein gene-product 9.5 (PGP9.5)—used as a panneuronal marker and SP; All photographs have been made by overlapping of green and red fluorescent channels (green for PGP 9.5 and red for SP). Neurons immunopositive to a particular substance studied are indicated with arrows.
From: Palus K, Całka J.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 2019 Dec 18;21(1):15.
doi: 10.3390/ijms21010015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Ratanti substance P antibody, vlone NC1/34 (8450-0505) used to highlight substance P expressing nerve cells in porcine heart muscle by immunofluorescence.
Image caption:
Nerve fibers immunoreactive to substance P (SP) in the porcine heart apex under physiological conditions (a) and after administration of bisphenol A in a dosage 0.05 mg/kg body weight/day (b). Nerves containing particular active substances are indicated with arrows.
From: Makowska K, & Gonkowski S.
Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart.
Animals. 2021; 11(3):780.
doi: 10.3390/ani11030780
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to identify substance P expressing cells in mouse colon by immunofluorescence.
Image caption:
Distribution pattern of nerve cells immunoreactive to protein gene-product 9.5 (PGP 9.5)— used as pan neuronal marker and other substance studied: galanin (GAL), neuronal isoform of nitric oxide synthase (nNOS) or substance P (SP) in the myenteric plexus (MP) or submucous plexus (SmP) of mice distal colon under physiological conditions (A1,B1,C1) and after administration of bisphenol A in low (A2,B2,C2) and high dose (A3,B3,C3). The pictures are the result of the overlap of both stainings. The arrows are pointing neurons immunoreactive for studied substances.
From: Makowska, K.; Lepiarczyk, E.; Gonkowski, S.
The Comparison of the Influence of Bisphenol A (BPA) and Its Analogue Bisphenol S (BPS) on the Enteric Nervous System of the Distal Colon in Mice.
Nutrients 2023, 15, 200.
doi 10.3390/nu15010200
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label neuronal cells in porcine bladder by immunofluorescence
Image caption:
Nerve cells immunoreactive to protein gene product (PGP 9.5; used here as a pan-neuronal marker) (a) and substance P (SP) (b) in the intrinsic ganglia of the porcine urinary bladder trigone wall under the physiological condition (I), and after administration of low (II) and high (III) doses of BPA. SP-positive neurons are marked with arrows. The right column (c) was created by overlapping pictures (a,b).
From: Makowska K, Lech P, Gonkowski S.
Bisphenol A Effects on Neurons' Neurochemical Character in the Urinary Bladder Intramural Ganglia of Domestic Pigs.
Int J Mol Sci. 2023 Nov 27;24(23):16792.
doi: 10.3390/ijms242316792.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label neurons in porcine dorsal root ganglia by immunofluorescence.
Image caption:
Fast blue-positive neurons in the dorsal root ganglia (DRG) supplying the ileocecal valve (ICV) (indicated with arrows) immunoreactive to calcitonin gene related peptide (CGRP) (A), substance P (SP) (B) and galanin (GAL) (C).
From: Mikołajczyk A, Kozłowska A, Gonkowski S.
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures.
Int J Mol Sci. 2018 Aug 28;19(9):2551.
doi: 10.3390/ijms19092551.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti substance P antibody, clone NC1/34 (8450-0505) used to label neurons in porcine dorsal root ganglia by immunofluorescence.
Image caption:
Examples of dorsal root ganglia (DRG) neurons supplying the ileocecal valve (FB-positive, blue) (C,F), immunoreactive to tubulin (TUBB3 used here as a neuronal marker, green) (A,D) and substance P (SP) (B), galanin (GAL) (E)
From: Mikołajczyk A, Kozłowska A, Gonkowski S.
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures.
Int J Mol Sci. 2018 Aug 28;19(9):2551.
doi: 10.3390/ijms19092551.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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Image caption:
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From: Bulc M, Całka J, Jana B, Palus K.
Effect of different glyphosate doses on the chemical coding of neurons of the enteric nervous system of the porcine descending colon.
Folia Morphol (Warsz). 2023 Dec 11 [Epub ahead of print].
doi: 10.5603/fm.97761. .
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Substance P
- Product Type
- Monoclonal Antibody
- Clone
- NC1/34
- Isotype
- IgG2a
- Specificity
- Substance P
Antibody Quality and Performance Guarantee
| Rat anti substance P antibody, clone NC1/34 recognizes the COOH terminal region of substance P, an eleven amino acid polypeptide neurotransmitter derived by proteolytic cleavage of protachykinin-1. Substance P binds to and activates the neurokinin-1 receptor. Substance P is a highly conserved neurotransmitter expressed throughout the animal kingdom. It is highly expressed in the spinal cord dorsal horn and dorsal root ganglia, but not in the ventral horn (Ribeiro-da-Silva andT Hökfelt 2000). It is also expressed in non-neuronal cells including microglia and other immune cells. Substance P has a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction (Hökfelt 2004). Rat anti substance P antibody, clone NC1/34 demonstrates ~5% reactivity with eledoisin a neurotransmitter identified in the octopus Genus Eledone. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin in tested applications. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Pig Pigeon Bichir Rabbit Human Bovine Guinea Pig Calanus sp. Crustacea Expected from Sequence Cancer sp. Panilurus sp. Homarus sp. Mudpuppy Lamprey Crayfish Zebrafish Bullfrog Amphibia Expected from Sequence Cockroach Sheep Cowbird Rat Spadefoot toad Chicken Chinchilla Camel Beaver Chinchilla - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Substance P conjugated to bovine serum albumin.
- Fusion Partners
- Spleen cells from immunized Wistar rat were fused with cells of the NS1/1-Ag 4-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
1/100 | 1/200 |
| Immunohistology - Paraffin 1 | |
1/100 | 1/200 |
- 1This product does not require antigen retrieval prior to staining of formalin fixed, paraffin embedded sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Substance P antibody
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Kozsurek, M. et al. (2009) Nonselective innervation of lamina I projection neurons by cocaine- and amphetamine-regulated transcript peptide (CART)-immunoreactive fibres in the rat spinal dorsal horn.
Eur J Neurosci. 29: 2375-87. -
Cuello, A.C. et al. (1980) Use of monoclonal antibodies in immunocytochemistry with special reference to the central nervous system.
Brain Res Bull. 5 (5): 575-87. -
Pioro, E.P. et al. (1985) Loss of substance P immunoreactivity in the nucleus of the spinal trigeminal tract after intradural tumour compression of the trigeminal nerve.
Neurosci Lett. 58: 7-12. -
Mai, J.K. et al. (1986) Substance P in the human brain.
Neuroscience. 17 (3): 709-39. -
Cuello, A.C. et al. (1979) Detection of substance P in the central nervous system by a monoclonal antibody.
Proc Natl Acad Sci U S A. 76 (7): 3532-6. -
Czujkowska, H. & Arciszewski, M.B. (2011) Expression of corticotropin releasing factor (CRF) in the enteric nervous system of the jejunum of sheep
Veterinarni medicina 56: 551-60. -
Kato, A. et al. (2012) Endocannabinoid-dependent plasticity at spinal nociceptor synapses.
J Physiol. 590 (Pt 19): 4717-33. -
Arciszewski, M.B. et al. (2009) Expression of vasoactive intestinal polypeptide, substance P and neuropeptide Y in jejunal enteric nerves is altered in rabbits suffering from long term Trichinella spiralis infection: an immunohistochemical study
Veterinarni Medicina, 54: 589–97
View The Latest Product References
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Bulc, M. et al. (2013) Immunohistochemical characterization of the porcine nodose ganglion.
Acta Histochem. 115 (5): 440-6. -
Boorsma, D.M. et al. (1982) Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance P.
J Histochem Cytochem. 30: 1211-6. -
Pioro, E.P. et al. (1984) Loss of substance P and Enkephalin immunoreactivity in the human substantia nigra after striato-pallidal infarction
Brain Res. 292: 339-47. -
Mortimer, S.T. et al. (1990) Immunohistochemical identification of calcitonin gene-related peptide and substance P in nerves of the bovine parathyroid gland.
Cell Tissue Res. 261: 339-45. -
Galligan, J.J. et al. (1988) Changes in surviving nerve fibers associated with submucosal arteries following extrinsic denervation of the small intestine.
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Horn, J.P. and Stofer, W.D. (1989) Preganglionic and sensory origins of calcitonin gene-related peptide-like and substance P-like immunoreactivities in bullfrog sympathetic ganglia.
J Neurosci. 9: 2543-61. -
Verhaert, P. & De Loof, A. (1985) Substance P-like immunoreactivity in the central nervous system of the blattarian insect Periplaneta americana L. revealed by a monoclonal antibody.
Histochemistry. 83: 501-7. -
Polgár, E. et al. (2007) A population of large neurons in laminae III and IV of the rat spinal cord that have long dorsal dendrites and lack the neurokinin 1 receptor.
Eur J Neurosci. 26: 1587-98. -
Nowicki, M. et al. (2007) Peptidergic and nitrergic innervation of the pineal gland in the domestic pig: an immunohistochemical study.
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Nair-Roberts, R.G. et al. (2006) Distribution of substance P reveals a novel subdivision in the hippocampus of parasitic South American cowbirds.
J Comp Neurol. 496: 610-26. -
Song, Y. et al. (2013) Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis.
BMC Musculoskelet Disord. 14: 134. -
Szczurkowski, A. et al. (2013) Morphology and immunohistochemical characteristics of the pterygopalatine ganglion in the chinchilla (Chinchilla laniger, Molina).
Pol J Vet Sci. 16 (2): 359-68. -
Pidsudko, Z. (2014) Immunohistochemical characteristics and distribution of sensory dorsal root Ganglia neurons supplying the urinary bladder in the male pig.
J Mol Neurosci. 52 (1): 71-81. -
Zacharko-Siembida, A. et al. (2014) Co-expression patterns of cocaine- and amphetamine-regulated transcript (CART) with neuropeptides in dorsal root ganglia of the pig.
Acta Histochem. 116 (2): 390-8. -
Dudek, A. et al. (2012) Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa's ganglion) of the pig.
Pol J Vet Sci.15: 499-507. -
Ibrahim, D. et al. (2015) Immunohistochemical studies for the neuronal elements in the vomeronasal organ of the one-humped camel.
J Vet Med Sci. 77 (2): 241-5. -
Christensen, J. et al. (2015) Protease-activated receptors in the Achilles tendon-a potential explanation for the excessive pain signalling in tendinopathy.
Mol Pain. 11: 13. -
Bossowska, A. et al. (2015) Botulinum Toxin Type A Induces Changes in the Chemical Coding of Substance P-Immunoreactive Dorsal Root Ganglia Sensory Neurons Supplying the Porcine Urinary Bladder.
Toxins (Basel). 7 (11): 4797-816. -
Sienkiewicz, W. et al. (2015) Innervation of the chinchilla testis, epididymis, and vas deferens
Bull Vet Inst Pulawy. 59 (4): 547-56. -
Czujkowska, A. & Arciszewski, M.B. (2016) Galanin is Co-Expressed with Substance P, Calbindin and Corticotropin-Releasing Factor (CRF) in The Enteric Nervous System of the Wild Boar (Sus scrofa) Small Intestine.
Anat Histol Embryol. 45 (2): 115-23. -
Klimczuk, M. et al. (2016) Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland.
Veterinarni Medicina. 61: 361-73. -
Gańko, M. & Całka, J. (2014) Localization and chemical coding of the dorsal motor vagal nucleus (DMX) neurons projecting to the porcine stomach prepyloric area in the physiological state and after stomach partial resection.
J Mol Neurosci. 52 (1): 90-100. -
Pidsudko, Z. et al. (2008) Distribution and chemical coding of intramural neurons in the porcine ileum during proliferative enteropathy.
J Comp Pathol. 138 (1): 23-31. -
Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery.
Anat Histol Embryol. 36 (3): 186-93. -
Gańko M & Całka J (2014) Prolonged acetylsalicylic-acid-supplementation-induced gastritis affects the chemical coding of the stomach innervating vagal efferent neurons in the porcine dorsal motor vagal nucleus (DMX).
J Mol Neurosci. 54 (2): 188-98. -
Dudek, A. et al. (2017) Chemical Coding of Sensory Neurons Supplying the Hip Joint Capsule in the Sheep.
Anat Histol Embryol. 46 (2): 121-31. -
Zacharko-Siembida, A. et al. (2017) An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
Acta Veterinaria. 67 (3): 397-408. -
Rytel, L. et al. (2018) Neurochemical characterization of intramural nerve fibres in the porcine oesophagus.
Anat Histol Embryol. 47 (6): 517-26. -
Zalecki, M. (2019) Gastric ulcer induced changes in substance P and Nk1, Nk2, Nk3 receptors expression in different stomach localizations with regard to intrinsic neuronal system.
Histochem Cell Biol. 151 (1): 29-42. -
Zalecki, M. et al. (2019) Enteric nervous system in the European beaver (Castor fiber) pylorus - an immunohistochemical study.
Pol J Vet Sci. 22 (1): 101-7. -
Sienkiewicz, W. et al. (2015) Caudal mesenteric ganglion in the sheep - macroanatomical and immunohistochemical study.
Pol J Vet Sci. 18 (2): 379-89. -
Sickinger, M. et al. (2020) Neuropeptides in the urinary tract of male sheep lambs.
Res Vet Sci. 133: 307-12. -
Szymanska, K. et al. (2018) The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 19(3 ): 917. -
Rytel, L. & Całka, J. (2020) Aspirin Administration Affects Neurochemical Characterization of Substance P-Like Immunoreactive (SP-LI) Nodose Ganglia Neurons Supplying the Porcine Stomach.
Biomed Res Int. 2020: 1049179. -
Palus, K. & Całka, J. (2019) Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 21 (1): 15. -
Makowska, K. & Gonkowski, S. (2021) Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart
Animals. 11 (3): 780. -
Bulc, M. et al. (2019) Endometritis affects chemical coding of the dorsal root ganglia neurons supplying uterus in the sexually mature gilts.
Res Vet Sci. 124: 417-25. -
Bulc, M. et al. (2018) Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes.
J Diabetes Res. 2018: 4735659. -
Palus, K. et al. (2019) The impact of low and high doses of acrylamide on the intramural neurons of the porcine ileum.
Food Chem Toxicol. 132: 110673. -
Meregalli, C. et al. (2021) Reversal of Bortezomib-Induced Neurotoxicity by Suvecaltamide, a Selective T-Type Ca-Channel Modulator, in Preclinical Models.
Cancers (Basel). 13 (19): 5013. -
Palus, K. et al. (2018) Changes in VIP-, SP- and CGRP- like immunoreactivity in intramural neurons within the pig stomach following supplementation with low and high doses of acrylamide.
Neurotoxicology. 69: 47-59. -
Makowska, K. et al. (2022) The Comparison of the Influence of Bisphenol A (BPA) and Its Analogue Bisphenol S (BPS) on the Enteric Nervous System of the Distal Colon in Mice
Nutrients. 15 (1): 200. -
Sienkiewicz, W. et al. (2023) Morphology and immunohistochemical characteristics of the otic ganglion in the chinchilla (Chinchilla laniger Molina).
Folia Histochem Cytobiol. 61 (1): 17-25. -
Benoit, L.B. et al. (2023) RBP Image Database: A resource for the systematic characterization of the subcellular distribution properties of human RNA binding proteins.
Nucleic Acids Res. 51 (D1): D1549-D1557. -
Bulc, M. et al. (2022) Administration of Different Doses of Acrylamide Changed the Chemical Coding of Enteric Neurons in the Jejunum in Gilts.
Int J Environ Res Public Health. 19 (21): 14514. -
Makowska, K. & Gonkowski, S. (2023) Changes Caused by Bisphenols in the Chemical Coding of Neurons of the Enteric Nervous System of Mouse Stomach.
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Makowska, K. et al. (2023) Bisphenol A Effects on Neurons' Neurochemical Character in the Urinary Bladder Intramural Ganglia of Domestic Pigs.
Int J Mol Sci. 24 (23): 16792. -
Ismail, H.I. & Ali, A.M. (2015) Immunohistochemical Studies on The Endocrine Cells in The Thymus of The One-Humped-Camel (Camelus dromedarius)
J Camel Pract Res. 22 (2): 251. -
Księżarczyk, M.M. & Arciszewski, M.B. (2021) Immunohistochemical study on the expressions of neuropeptides in the superficial and deep gland of the third eyelid of pigs.
Anat Histol Embryol. 50 (3): 579-87. -
Sienkiewicz, W. et al. (2022) Immunohistochemical characterization of nerve elements in porcine intrinsic laryngeal ganglia.
Pol J Vet Sci. 25 (2): 325-34. -
Rumpler, É. et al. (2021) Characterization of Kisspeptin Neurons in the Human Rostral Hypothalamus.
Neuroendocrinology. 111 (3): 249-62. -
Mikołajczyk, A. et al. (2018) Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures.
Int J Mol Sci. 19 (9): 2551. -
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Folia Morphol (Warsz). Dec 11 [Epub ahead of print].
- RRID
- AB_2200292
- UniProt
- P20366
- Entrez Gene
- TAC1
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