Detection of biotinylated Goat anti Hamster IgG (STAR79B) in a flow cytometric analysis of EMR1 expression on human eosinophils with Hamster anti Human EMR1(MCA2674) as the primary detection antibody and Streptavidin:RPE (STAR4A) as the tertiary reagent
Streptavidin conjugated to R-Phycoerythrin - lyophilised
Reconstitute with 1ml distilled water
Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.
STAR4A conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.
Bovine Serum Albumin
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of reconstitution.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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