Streptavidin

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Streptavidin gallery image 1

Published customer image:
biotin cinjugated Goat anti Mouse IgG (103008) used in conjunction with horseradish peroxidase conjugated streptavidin (710005) for the detection of anti tumor derived antigen IgG by ELISA.
Image caption:
Local melanoma tumors generate systemic circulating IgG levels. (A). Outline of local tumor injection experiment. B16.F10-tdTom+ cells were s.c. injected into C57BL/6 mice on day 0 and allowed to proliferate for 30 days. In some mice, B16.F10-tdTom lysate was additionally s.c. injected to test whether IgG production can be boosted. (B) Brightfield (top) and red fluorescent (bottom) stereomicroscopic images of tumor-injected and contralateral footpads on day 30. (C) IgG titers in control and tumor-bearing mice on day 30, with and without additional B16.F10-tdTom+ cell lysate injection for 15 days. Each dot represents the serum of one mouse. Data in (C) are pooled from two independent experiments and were analyzed by a Kruskal–Wallis test with Dunn’s multiple comparison test. **p < 0.01, ***p < 0.001.

From: F. Moalli, S. T. Proulx, R. Schwendener, M. Detmar, C. Schlapbach, and J. V. Stein, “Intravital and Whole-Organ Imaging Reveals Capture of Melanoma-Derived Antigen by Lymph Node Subcapsular Macrophages Leading to Widespread Deposition on Follicular Dendritic Cells,” Front. Immunol., vol. 6, Mar. 2015.

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  • Streptavidin thumbnail image 1
  • Streptavidin:RPE-Cy7
  • Streptavidin:HRP
  • Streptavidin:Alk. Phos.
  • Streptavidin:RPE-Cy5.5
  • Streptavidin:FITC
  • Streptavidin:RPE-Cy7
  • Streptavidin:Texas Red®
  • Streptavidin:RPE-Cy5.5
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  • Product Type
    Accessory Reagent
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    710017SFdatasheet pdfdatasheet pdf0.1 mg
    710017S
    710016SFdatasheet pdfdatasheet pdf0.1 mg
    710016S
    710017LFdatasheet pdfdatasheet pdf0.5 mg
    710017L
    710016LFdatasheet pdfdatasheet pdf0.5 mg
    710016L
    710002Fdatasheet pdfdatasheet pdf1 mg
    710002
    710007Fdatasheet pdfdatasheet pdf1 mg
    710007
    710005C, E, IBdatasheet pdfdatasheet pdf1 ml
    710005
    710004C, E, IBdatasheet pdfdatasheet pdf1 ml
    710004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Product Form
      Recombinant Protein - conjugated to RPE/ Cy7 - liquid
      Purified Protein conjugated to Horseradish Peroxidase (HRP) - liquid
      Protein conjugated to Alkaline Phosphatase - liquid
      Recombinant Protein - conjugated to RPE/ Cy5.5 - liquid
      Recombinant Protein - conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Recombinant Protein - conjugated to RPE/ Cy7 - liquid
      Recombinant Protein conjugated to Texas Red® (TXRD) - Liquid
      Recombinant Protein - conjugated to RPE/ Cy5.5 - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium azide
      Stabilising agent
      50% glycerol.
      0.1% Sodium Azide (NaN3) 50% glycerol
      0.09% Sodium azide
      Stabilising agent
      0.1% Sodium Azide (NaN3)
      0.09% Sodium azide
      Stabilising agent
      0.1% Sodium azide
      0.09% Sodium azide
      Stabilising agent
    • Purity
    • Approx. Protein Concentrations
      0.5 mg/ml
      0.5 mg/ml
      1.0 mg/ml
      0.5 mg/ml
      1.0 mg/ml
      0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mg+4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      Pack Size: 0.5 mg+4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      +4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      +4ºC, -20ºC if preferred
      This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the protein.
      Pack Size: 0.1 mg+4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      Pack Size: 0.5 mg+4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      +4ºC, -20ºC if preferred
      This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      +4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
    • Shelf Life
      Please see label for expiry date.
      12 months from date of despatch.
      18 months from date of despatch.
      6 months from the date of despatch.
      6 months from the date of despatch.
      Please see label for expiry date.
      6 months from the date of despatch.
      Please see label for expiry date.
    • Acknowledgements
      Cy® and CyDye® are registered trademarks of GE Healthcare
      Cy® and CyDye® are registered trademarks of GE Healthcare
      Cy® and CyDye® are registered trademarks of GE Healthcare
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/4,0001/8,000
      Immunoblotting
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2,0001/4,000
      Immunoblotting
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Streptavidin Formats

    Formats Applications Sizes available
    Streptavidin : RPE-Cy7 F 0.1 mg | 0.5 mg
    Streptavidin : HRP C, E, IB 1 ml
    Streptavidin : RPE-CY5.5 F 0.5 mg | 0.1 mg
    Streptavidin : Alk. Phos. C, E, IB 1 ml
    Streptavidin : Texas Red® F 1 mg
    Streptavidin : FITC F 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A

          Recommended Positive Controls

            Histology Controls

              References

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