Prolactin Antibody

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Prolactin Antibody gallery image 1

Published customer image:
Rabbit antiSheep prolactin antibody (7770-0104) used for the evaluation of prolactin content of secreted microvesicles by western blotting.
Image caption:
RHBDL4-induced stabilization of higher molecular weight forms leads to their detection in secreted microvesicles.
(a) proTGFα-FLAG or CD44-FLAG were co-expressed in Hek293T cells with RHBDL4 (R4) or the catalytically inactive RHBDL4 mutant (R4-SA) in presence of 10 μM BB94. After removing cell debris, conditioned media were subjected to ultracentrifugation. The 37-kDa form of proTGFα-FLAG and a subpopulation of CD44-FLAG were recovered in the 175,000?×?g pellet fraction (p), whereas the trimmed FLAG-tagged TGFα pro-peptide (indicated by asterisk) was in the supernatant fraction (sn). t, total cell extracts. (b) proTGFα and CD44 from the 175,000?×?g pellet fraction as obtained in (a) float in a sucrose gradient indicating that they are secreted by microvesicles. Soluble secreted prolactin serves as negative control as it is found in the supernatant (sn) fraction only. Sucrose density ranges from 1.06 g/l for the top (fraction 1) to 1.25 g/l (fraction 9); sp, sucrose gradient pellet. (c) Released proTGFα-FLAG (black arrow) is trapped by a His-tag that has been introduced between TACE-cleavage site and the juxtamembrane region. In contrast, FLAG-tagged pro-peptide (asterisk) is not bound.

From: Wunderle L, Knopf JD, Kühnle N, Morlé A, Hehn B, Adrain C, Strisovsky K, Freeman M, Lemberg MK.
Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFα.
Sci Rep. 2016 Jun 6;6:27342.

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Prolactin Antibody gallery image 2

Published customer image:
Rabbit antiSheep prolactin antibody (7770-0104) used for the evaluation of prolactin content of Hek293 cell lysates by western blotting.
Image caption:
RHBDL4-mediated proTGFα-secretion boost is enhanced by transactivation. (a) Exosome secretion of proTGFα-FLAG in COS7 cells ectopically expressing GRPR is stimulated by PMA or bombesin (bbs), mimicking the effect caused by RHBDL4 overexpression. In absence of BB94 (BB), transactivation by PMA and bbs also affects the extent of pro-peptide cleavage subsequently reducing the amount of secreted pro-peptide (asterisk) in the media fraction. (b) Upon transactivation of COS7 cells with bbs, the secreted 37-kDa form of proTGFα-FLAG was processed to the 6-kDa mature form via a number of intermediates. Of note, due to a low expression the ER-resident 28-kDa form of proTGFα-FLAG was not detected (cf. Fig. 1a,g). (c) Time course of TGFα secretion after PMA stimulation of Hek293T ectopically expressing proTGFα-FLAG was performed in presence of BB94 (BB, 20 μM), DCI (100 μM) or both. (d) PMA and bbs-induced proTGFα-FLAG secretion boost can be suppressed by RHBDL4 knockdown in Hek293 T-REx cells expressing an inducible shRNA but not in control cells. This effect is rescued by ectopically expressed mouse RHBDL4 (mRHBDL4-HA). Cytosolic GFP and ectopically expressed prolactin (Prl) serve as transfection and loading controls.

From: Wunderle L, Knopf JD, Kühnle N, Morlé A, Hehn B, Adrain C, Strisovsky K, Freeman M, Lemberg MK.
Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFα.
Sci Rep. 2016 Jun 6;6:27342.

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  • Rabbit anti Sheep Prolactin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7770-0104E, IF, Pdatasheet pdfdatasheet pdf50 µl
    7770-0104
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti sheep PRL antibody recognizes ovine prolactin, a 229 amino acid ~23 kDa somatotropin that acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation (UniProt: P01240). Prolactin can also exist in an alternative glycosylated form increasing it's apparrent molecular weight to ~25 kDa (Lewis et al. 1984). Glycosylation also appears to negatively affect the potency of ovine prolactin in a variety of explant bioassays (Markoff et al. 1988).

      Rabbit anti sheep PRL antibody has been successfully used for the detection of prolactin in plasma of mice by ELISA (Hartwell et al. 2014) and western blotting (Wunderle et al. 2016).
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Catyes
      Humanyes
      Dogyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Native, from pituitary
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      lactation
      hormone activity
      extracellular region
      prolactin receptor binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence1/201/40
      Immunohistology - Paraffin1/5001/3K

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      In a simple ELISA, with 400 ng of antigen coated per well, the 50% OD was 1/40K. The end point titre was observed at an antibody dilution of 1/5M, under the same conditions.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Prolactin Antibody Formats

    Formats Applications Sizes available
    Prolactin Antibody : Serum E, IF, P 50 µl
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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