MHC Class II DR Monomorphic antibody | 37.68
FITC conjugated Mouse anti Sheep MHC class II antibody, clone 37.68 (MCA2226F) used to evaluate MHC class II expression on goat monocytes by flow cytometry.
Image caption:
rFg-CaBP4-mediated differential expression of major histocompatibility complex (MHC) on the surface of goat monocytes. Monocytes were cultured in the presence of the indicated concentrations of rFg-CaBP4 and control buffer for 24 h. MHC class-I (MHC-I) and MHC-II expressions were measured by flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI). The data are presented as the MFI ± SD from three independent experiments. **P <0.01, ***P <0.001, ****P <0.0001, ns (compared to untreated control)
From: Ehsan M, Hu RS, Hou JL, Elsheikha HM, Li XD, Liang PH, Zhu XQ.
Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 2021 May 22;14(1):276.
doi: 10.1186/s13071-021-04784-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Fitc conjugated Mouse anti Sheep MHC Class II antibody, clone 37.68 (MCA2226F) used to evaluate MHC Class II expression in goat monocytes by flow cytometry.
Image caption:
Inhibitory effect of recombinant rhodanese protein from Haemonchus contortus (rHCRD) on the expression of MHC-II by goat monocytes. Cells were cultured in the presence of varies rHCRD concentrations and his-tagged protein or control buffer (PBS/DTT) for 24 h. The cells treated with LPS were used as positive control. MHC-II expression was analysed on flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI) of controls. Bars represent the MFI ± SD of controls. The data presented are results of three independent experiments (*P <0.05, **P <0.01, ***P <0.001). a MHC-I. b MHC-II
From: Wang Y, Ehsan M, Huang J, Aimulajiang K, Yan R, Song X, Xu L, Li X.
Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 2020 Sep 7;13(1):454.
doi: 10.1186/s13071-020-04333-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep MHC Class II DR Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- 37.68
- Isotype
- IgG2a
- Specificity
- MHC Class II DR Monomorphic
Antibody Quality and Performance Guarantee
| Mouse anti Sheep MHC class II antibody, clone 37.68 recognizes a monomorphic epitope on ovine MHC class II DR molecules, constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Splenocytes from ATL mice.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
Source Reference
-
Puri, N.K. et al. (1985) Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.
Immunology. 56 (4): 725-33.
References for MHC Class II DR Monomorphic antibody
-
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet Immunol Immunopathol. 15 (1-2): 59-86. -
Puri, N.K. & Brandon, M.R. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.
Immunology. 62 (4): 575-80. -
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
Hum Immunol. 20 (3): 195-207. -
Ballingall, K.T. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants.
Anim Genet. 26 (2): 79-84. -
Wang, Y. et al. (2017) Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytes.
Parasit Vectors. 10 (1): 425. -
Wang, Y. et al. (2020) Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 13 (1): 454. -
López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
Tissue Eng Part A. 26 (17-18): 993-1004. -
Ehsan, M. et al. (2021) Fasciola gigantica. tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 14 (1): 276.
View The Latest Product References
- RRID
- AB_324580
MCA2226
0312
157534
167457
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