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FITC conjugated Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 (MCA2228F) used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence.
Differential host-virus interactions in nasopharyngeal mucosa of non-vaccinated (A-C) versus vaccinated (D-F) cattle. Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). Host cells expressing MHC II (purple) and/or CD11c (aqua) are present in the subepithelial compartment and interspersed within epithelium, but without co-localizing with viral protein. 10x magnification, scale bar 100μm. B-C) 40x magnification of region identified in (A), scale bars 25μm. B) Select channel views demonstrate lack of co-localization of FMDV VP1 (red) with CD11c (aqua)/ MHC II (purple)-double positive cells. C) Merge of all images shown in (B), scale bar 25 μm. D) FMDV VP1 (red) protein co-localizes with cytokeratin (green) in an epithelial erosion in dome region of follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). Regionally extensive infiltration by MHC II (purple) and/or CD11c (aqua)-expressing cells co-localizing and interspersing with viral antigen.10x magnification, scale bar 100μm. E-F) 40x magnification of region identified in (D), scale bar 25μm. E) Select channels demonstrating co-localization of FMDV VP1 (red) with CD11c (aqua), MHC II (purple). F) Merge of images shown in (E), scale bar 25 μm.
From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666.