MHC Class II DQ DR Polymorphic Antibody | 49.1

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MHC Class II DQ DR Polymorphic Antibody | 49.1 gallery image 1

Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR (POLYMORPHIC) (MCA2228F)

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MHC Class II DQ DR Polymorphic Antibody | 49.1 gallery image 2

Published customer image:
Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence.
Image caption:
Primary infection of nasopharyngeal mucosal epithelial cells is similar in non-vaccinated and vaccinated cattle.
Microscopic distribution of FMDV structural (VP1) and non-structural (3D) protein in dorsal nasopharyngeal mucosa of non-vaccinated (A-C) and vaccinated (D-F) cattle at 24 hpi. Multichannel immunofluorescent technique. A) Nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). FMDV VP1 (red) and 3D (aqua) proteins co-localize with cytokeratin (green) in foci of primary FMDV infection in superficial layer of follicle-associated epithelium. Scarce MHC II+ cells (purple) are present in the subepithelium. 20x magnification, scale bar 50μm. B-C) 40x magnification of region identified in (A). B) Select channel combinations demonstrating co-localization of FMDV VP1(red) and FMDV 3D (aqua) with cytokeratin (green), but not with MHC II (purple) C) Merge of all images shown in (B), scale bar 25μm. D) Nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). FMDV VP1 (red) and 3D (aqua) proteins co-localize with cytokeratin (green) within follicle associated epithelium. Intra-epithelial MHC II+ cells (purple) are in close proximity to virus-infected epithelial cells. 20x magnification, scale bar 50μm. E-F) 40x magnification of region identified in (D). E) Select channel combinations demonstrating FMDV VP1 (red) and 3D (aqua) colocalization with cytokeratin (green)-expressing cells and with MHC II- expressing cells (purple). E) Merge of images shown in (F), scale bar 25μm.

From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666.

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MHC Class II DQ DR Polymorphic Antibody | 49.1 gallery image 3

Published customer image:
Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence.
Image caption:
Differential host-virus interactions in nasopharyngeal mucosa of non-vaccinated (A-C) versus vaccinated (D-F) cattle. Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). Host cells expressing MHC II (purple) and/or CD11c (aqua) are present in the subepithelial compartment and interspersed within epithelium, but without co-localizing with viral protein. 10x magnification, scale bar 100μm. B-C) 40x magnification of region identified in (A), scale bars 25μm. B) Select channel views demonstrate lack of co-localization of FMDV VP1 (red) with CD11c (aqua)/ MHC II (purple)-double positive cells. C) Merge of all images shown in (B), scale bar 25 μm. D) FMDV VP1 (red) protein co-localizes with cytokeratin (green) in an epithelial erosion in dome region of follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). Regionally extensive infiltration by MHC II (purple) and/or CD11c (aqua)-expressing cells co-localizing and interspersing with viral antigen.10x magnification, scale bar 100μm. E-F) 40x magnification of region identified in (D), scale bar 25μm. E) Select channels demonstrating co-localization of FMDV VP1 (red) with CD11c (aqua), MHC II (purple). F) Merge of images shown in (E), scale bar 25 μm.

From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666.

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MHC Class II DQ DR Polymorphic Antibody | 49.1 gallery image 4

Published customer image:
Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence.
Image caption:
FMDV co-localizes with cytokeratin and MHCII/CD11c-expressing cells in palatine tonsil of non-vaccinated steer at 72hpi.
A) FMDV VP1 (red) predominantly in cytokeratin-positive cells of epithelial crypt of palatine tonsil of non-vaccinated steer (animal number 9) during viremia and clinical FMD at 72 hpi. Abundant cells expressing MHC II (purple) and/or CD11c (aqua) interspersed with epithelial cells. 10x magnification, scale bar 100 μm. B) Select channels of image in (A). FMDV VP1 (red), cytokeratin (green), CD11c (aqua), MHC II (purple) demonstrate FMDV antigen is exclusively within epithelial compartment. C) High magnification of region identified in (A). Co-localization with FMDV VP1within subset of MHC II (purple) and/or CD11c (aqua) expressing cells within crypt epithelium (arrows in C & D). 40x magnification, scale bar 25 μm D) Individual channels of image in (B). FMDV VP1 (red), cytokeratin (green), CD11c (aqua), MHC II (purple).

From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666.

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  • Product Type
    Monoclonal Antibody
  • Clone
    49.1
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2228FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2228F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules, constitutively expressed on antigen presenting cells including dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

      The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infection. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.

      Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 recognizes ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1 (Ballingall et al. 1995).
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      Humanyes
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MHC Class II DQ DR Polymorphic Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class II DQ DR Polymorphic Antibody : FITC 49.1 F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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