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Phycoerythrin conjugated Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 (MCA2225PE) used for the identification of MHC class II expressing cells in bovine nasopharyngeal tissue sections by immunofluorescence.
Immunofluorescent detection of persistent FMDV in nasopharyngeal mucosa. Dorsal nasopharynx, steer #626, 37dpe, FMDV O1 Manisa. Immunomicroscopy, A) Low magnification (10X) view of an epithelial invagination with a focal cluster of FMDV-antigen-positive cells within superficial epithelial surface, scale bar 50 µm. B-F) Higher magnification (40X) views of region of interest identified in A (dashed box). Cells containing FMDV-VP1 are in the superficial epithelium and are cytokeratin-positive. Few MHC-II and CD11c positive cells are present within and below epithelium, but do not contain FMDV-VP1. Indirect fluorescence technique with differential interference contrast, antibody labels color-coded in bottom panel, asterisks indicate channels present in each panel, scale bar 25 µm.
From: Pacheco JM, Smoliga GR, O’Donnell V, Brito BP, Stenfeldt C, Rodriguez LL, et al. (2015) Persistent Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Cattle; Tissue-Specific Distribution and Local Cytokine Expression. PLoS ONE 10(5): e0125698.