MHC Class II DQ DR Polymorphic Antibody | 28.1

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MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 1

Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR:FITC (MCA2225F)

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MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 2

Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR:RPE (MCA2225PE)

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MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 3

Published customer image:
Phycoerythrin conjugated Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 (MCA2225PE) used for the identification of MHC class II expressing cells in bovine nasopharyngeal tissue sections by immunofluorescence.
Image caption:
Immunofluorescent detection of persistent FMDV in nasopharyngeal mucosa. Dorsal nasopharynx, steer #626, 37dpe, FMDV O1 Manisa. Immunomicroscopy, A) Low magnification (10X) view of an epithelial invagination with a focal cluster of FMDV-antigen-positive cells within superficial epithelial surface, scale bar 50μm. B-F) Higher magnification (40X) views of region of interest identified in A (dashed box). Cells containing FMDV-VP1 are in the superficial epithelium and are cytokeratin-positive. Few MHC-II and CD11c positive cells are present within and below epithelium, but do not contain FMDV-VP1. Indirect fluorescence technique with differential interference contrast, antibody labels color-coded in bottom panel, asterisks indicate channels present in each panel, scale bar 25 µm.

From: Pacheco JM, Smoliga GR, O'Donnell V, Brito BP, Stenfeldt C, Rodriguez LL, et al. (2015)
Persistent Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Cattle; Tissue-Specific Distribution and Local Cytokine Expression.
PLoS ONE 10(5): e0125698.

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MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 4

Published customer image:
Phycoerythrin conjugated Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC class II expression in palatine tonsil of FMDV infected cattle by immunofluorescence on cryostat tissue sections.
Image caption:
FMDV generates microvesicles within the palatine tonsil of cattle during the clinical phase of disease. a Low magnification image demonstrates the architecture of a large tonsillar crypt delineated by cytokeratin-positive (green) squamous epithelium. Dashed box indicates region of interest (ROI) within crypt wall containing a focus of FMDV (red)-infected cells forming a microvesicle. ROI is featured at higher magnification in 6b-d including different detection channels in each panel. b Inclusion of FMDV VP1 (red) and cytokeratin (green) channels demonstrates disruption of epithelial architecture with cavitation/vesiculation. Many of the FMDV-containing cells are also cytokeratin-positive (epithelial cells). c Inclusion of FMDV VP1 (red), CD11c (turquoise), and MHC II (purple) demonstrates that some of the FMDV-containing cells are also individually- or double-positive for these markers of monocytoid, phagocytic, and antigen presenting cells. d Simultaneous viewing of all 4 channels demonstrates that within the vesicular cavity, FMDV-containing cells of distinct phenotypes are interspersed and in close proximity. Multichannel immunofluorescence microcopy. (animal ID 938, tissue ID palatine tonsil) (magnification: a 4×, b-d 40×).

From: Arzt J, Pacheco JM, Stenfeldt C, Rodriguez LL.
Pathogenesis of virulent andattenuated foot-and-mouth disease virus in cattle.
Virol J. 2017 May 2;14(1):89.

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MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 5

Published customer image:
Phycoerythrin conjugated Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC class II expression in palatine tonsil of FMDV infected cattle by immunofluorescence on cryostat tissue sections.
Image caption:
FMDV generates microvesicles within the palatine tonsil of cattle during the clinical phase of disease. a Low magnification image demonstrates the architecture of a large tonsillar crypt delineated by cytokeratin-positive (green) squamous epithelium. Dashed box indicates region of interest (ROI) within crypt wall containing a focus of FMDV (red)-infected cells forming a microvesicle. ROI is featured at higher magnification in 6b-d including different detection channels in each panel. b Inclusion of FMDV VP1 (red) and cytokeratin (green) channels demonstrates disruption of epithelial architecture with cavitation/vesiculation. Many of the FMDV-containing cells are also cytokeratin-positive (epithelial cells). c Inclusion of FMDV VP1 (red), CD11c (turquoise), and MHC II (purple) demonstrates that some of the FMDV-containing cells are also individually- or double-positive for these markers of monocytoid, phagocytic, and antigen presenting cells. d Simultaneous viewing of all 4 channels demonstrates that within the vesicular cavity, FMDV-containing cells of distinct phenotypes are interspersed and in close proximity. Multichannel immunofluorescence microcopy. (animal ID 938, tissue ID palatine tonsil) (magnification: a 4×, b-d 40×).

From: Arzt J, Pacheco JM, Stenfeldt C, Rodriguez LL.
Pathogenesis of virulent andattenuated foot-and-mouth disease virus in cattle.
Virol J. 2017 May 2;14(1):89.

Enlarge
MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 6

Published customer image:
Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC II expression on CD1b+ ovine lymph cells by flow cytometry.
Image caption:
CD1b+ L-DCs are migratory mature DC capable of endocytic and phagocytic activities. After labelling lymph cells with anti- CD1b mAb (A), CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from 3 sheep (B). Plots of different markers expressed by CD1b+ L-DCs are shown (C). May-Grünwald Giemsa staining of sorted and cytocentrifugated CD1b+ L-DCs (D). Expression of mRNA for CCR7, CD103 and DC-SIGN by sorted CD1b+ L-DCs (1, 3, 5 respectively) and in cDNA control (2, 4, 6 respectively) (E). Uptake of FITC-ovalbumin (FITC-OVA) or GFP-Salmonella by CD1b+ L- DCs. Lymph cells (1×106 cells) were incubated with FITC-OVA for 1h at 4°C or 37°C, or with GFP-Salmonella for 30 min at 37°C, followed by CD1b labelling. CD1b+ L- DC subset was gated on lymph cells and FITC-OVA fluorescence shown on dot plots F and G. Total L-APC were gated on SSC/FSC and the cell population negative for lymphocyte markers. Quadrants were then defined on the CD1b+ and CD1b- subsets and GFP-Salmonella fluorescence shown on dot plot H.

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.

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MHC Class II DQ DR Polymorphic Antibody | 28.1 gallery image 7

Published customer image:
Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC II expression on CD1b+ ovine lymph cells by flow cytometry.
Image caption:
Presentation of Salmonella antigens to CD4+ T cells by CD1b+ L-DCs primed by conjunctival vaccination with the Salmonella Rv-6 strain.
After labelling of cervical lymph cells with anti- CD1b mAb, CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from three sheep (A). Plots of different markers expressed by cervical CD1b+ L-DCs are shown (B). Sorted cervical CD1b+ L-DCs were isolated from sheep before and after conjunctival vaccination with Salmonella, and co-cultured with autologous purified CD4+ T cells at a ratio of 1:100. Cells were co-cultured for six days and the proliferative response assessed by [3H]-thymidine incorporation (B). Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. The results shown represent three independent experiments performed on one sheep. .

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.

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  • Product Type
    Monoclonal Antibody
  • Clone
    28.1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2225PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2225PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules, which are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

      The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.

      Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 recognises ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. (Ballingall, K. et al. 1995).
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Ovine alveolar macrophages.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system uising appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MHC Class II DQ DR Polymorphic Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class II DQ DR Polymorphic Antibody : RPE 28.1 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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