MHC Class I Monomorphic Antibody | 41.17

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MHC Class I Monomorphic Antibody | 41.17 gallery image 1

Staining of ovine peripheral blood lymphocytes with Mouse anti Sheep MHC Class I Monomorphic (MCA2224GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

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  • MHC Class I Monomorphic Antibody | 41.17 thumbnail image 1
  • Mouse anti Sheep MHC Class I Monomorphic
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  • Product Type
    Monoclonal Antibody
  • Clone
    41.17
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2224GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2224GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Sheep MHC Class I antibody, clone 41.17 recognizes a monomorphic determinant on the class I MHC. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC class I molecules are expressed at varying levels on the majority of nucleated cells.

      Mouse anti Sheep MHC Class I antibody, clone 41.17 will immunoprecipitate a band of approximately 44 kDa under reducing conditions and has also been reported to react with paraffin-embedded material following alcohol fixation.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Ovine lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS/11-Ag4-1 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MHC Class I Monomorphic Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class I Monomorphic Antibody : Purified 41.17 C, F, IP 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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