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Horseradish peroxidase conjugated Donkey anti Sheep/Goat IgG antibody used for the measurement of Tci-L4-ES-specific IgG responses in sheep by ELISA.
The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression.?Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta?L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 µg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A)?Faecal egg count (FEC) data over the course of the experimental infection. (B)?Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C)?Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta?infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a?significant difference between Con A + PBS vs. PBS-stimulated cultures; b?significant difference between ConA + ES vs. PBS-stimulated cultures; c?significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P?< 0.05, ** P?< 0.01, ***P?< 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).
From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..
From: McNeilly, T. et al. 2013 Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products. Veterinary Research 44:70