IgG Antibody

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IgG Antibody gallery image 1

Detection of Goat anti Human GADD34 (AHP683) in a Western blot analysis of HeLa whole cell lysate using Donkey anti Sheep/Goat IgG:HRP (STAR88P)

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IgG Antibody gallery image 2

Detection of Sheep anti Human alpha 1 microglobulin (AHP2149) in an antigen down ELISA analysis on purified alpha 1 microglobulin using Donkey anti Sheep/Goat IgG:HRP (STAR88P)

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IgG Antibody gallery image 3

Published customer image:
Dylight 488 conjugated donkey anti Sheep IgG antibody used for the visualization of bound sheep anti plasminogen antibody by immunofluorescence.
Image caption:
PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS was performed as described in "Materials and Methods". Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope.

From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 2010 Mar 12;10:76.

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IgG Antibody gallery image 4

Published customer image:
Insert meta-description and applications
Image caption:
Published customer image:
Horseradish peroxidase conjugated Donkey anti Sheep/Goat IgG antibody used for the measurement of Tci-L4-ES-specific IgG responses in sheep by ELISA.
Image caption:
The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression.?Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta?L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 µg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A)?Faecal egg count (FEC) data over the course of the experimental infection. (B)?Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C)?Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta?infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a?significant difference between Con A + PBS vs. PBS-stimulated cultures; b?significant difference between ConA + ES vs. PBS-stimulated cultures; c?significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P?< 0.05, ** P?< 0.01, ***P?< 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).

From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..

From: McNeilly, T. et al. 2013 Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products. Veterinary Research 44:70

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • Donkey anti Sheep/Goat IgG:Dylight®405
  • Donkey anti Sheep/Goat IgG:DyLight®488
  • Donkey anti Sheep/Goat IgG:DyLight®549
  • Donkey anti Sheep/Goat IgG:HRP
  • Donkey anti Sheep/Goat IgG:Alk. Phos.
  • Donkey anti Sheep/Goat IgG:DyLight®649
  • Donkey anti Sheep/Goat IgG:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
7 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR88D405GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR88D405GA
    STAR88D488GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR88D488GA
    STAR88D549GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR88D549GA
    STAR88D649GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR88D649GA
    STAR88AC, E, WBdatasheet pdfdatasheet pdf1 mg
    STAR88A
    STAR88FCdatasheet pdfdatasheet pdf1 mg
    STAR88F
    STAR88PC, E, P, WBdatasheet pdfdatasheet pdf1 ml
    STAR88P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Donkey anti Sheep/Goat IgG antibody recognizes both sheep and goat IgG, which are immunologically very similar and has been adsorbed against human, rabbit, mouse and rat serum to minimise cross-reactivity.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to DyLight®405 - liquid
      Purified IgG conjugated to DyLight®488 - liquid
      Purified IgG conjugated to DyLight®549 - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to DyLight®649 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Sheep IgG were raised by repeated immunisation of a donkey with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.05% Proclin™ 300
      0.002% Methylisothiazolone
      0.002% Bromonitrodioxane
      1% Bovine Serum Albumin
      0.01%Sodium Azide
      1%Bovine Serum Albumin
      50%Glycerol
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Purified IgG from a sheep serum pool.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      TRIS buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/128001/25600
      Immunohistology - Frozen1/501/100
      Immunohistology - Paraffin
      Western Blotting1/20000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/5000
      Immunohistology - Frozen
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1001/200

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Alk. Phos. C, E, WB 1 mg
    IgG Antibody : DyLight®488 F, IF 0.1 mg
    IgG Antibody : HRP C, E, P, WB 1 ml
    IgG Antibody : DyLight®649 F, IF 0.1 mg
    IgG Antibody : FITC C 1 mg
    IgG Antibody : DyLight®549 F, IF 0.1 mg
    IgG Antibody : DyLight®405 F, IF 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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