CD8 Antibody | 38.65

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CD8 Antibody | 38.65 gallery image 1

Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine CD8:FITC (MCA2216F)

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Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine CD8 MCA2216GA) followed by Rabbit F(ab')2 ANTI MOUSE IgG:FITC (STAR9B)

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CD8 Antibody | 38.65 gallery image 3

Published customer image:
Mouse anti Bovine CD8 antibody, clone 38.65 used to demonstrate the presence of CD8 expressing T cells in ovine placenta during gestation by immunohistochemistry on cryostat sections.
Image caption:
Comparison of the immunohistochemical labelling of T inflammatory cells and parasite antigen in the placenta. This panel compares the distribution and frequency of T lymphocytes (CD3, CD4 and CD8 antigens) and parasite antigen immunohistochemically labelled in samples from the three groups. Pictures showing the labelling of T cells (first three rows) were taken at 75× and those of the parasite antigen (last row) were taken at 150×. F: foetal mesenchyme, M: maternal endometrial stalk.

From: Arranz-Solís D, Benavides J, Regidor-Cerrillo J, Horcajo P, Castaño P, Del Carmen Ferreras M, Jiménez-Pelayo L, Collantes-Fernández E, Ferre I, Hemphill A, Pérez V, Ortega-Mora LM.
Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 2016 Jan 6;47(1):2.

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CD8 Antibody | 38.65 gallery image 4

Published customer image:
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine Peyer's patch and jejenum by flow cytometry and immunofluorescence.
Image caption:
CD8+ lymphocyte increase in the jejunum and jejunal Peyer's patches. (A) Cells isolated from jejunal Peyer's patches (JPP) and jejunum were double labelled with anti CD8 and NCR1 mAbs and analyzed by flow cytometry. The plots show data from a 6 dpi inoculated lamb and its 7 day-old matched control and are representative of 8 matched pairs of 7 day-old lambs. The gating used for the analyses shown in B is indicated with the corresponding colours. (B) The percentages of the 3 different populations of CD8+ lymphocytes corresponding to the 3 gates shown in (A) were analyzed in jejunum and JPP from lambs 2–6 dpi. Control lambs (open symbols), inoculated lambs (filled symbols). The means are indicated in red. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with * p?<?0.05, ** p?<?0.01, *** p?<?0.001. (C-D) Section of an ileal Peyer's patch (IPP) from a 7 day old control (C) and a 6 dpi lamb (D) were double labelled with anti CD8 (green) and Ki-67 (blue) antibodies. The images shown are obtained by merging these images with the white light image. The double positive cells (arrow) display CD8 cytoplasmic staining and Ki67 nuclear staining. Pinpoint sized spots (arrowhead) are identified as autofluorescence. Dome (d), lamina propria (lp). Bar 50 μm.

From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment".
Veterinary Research 2015 46:28.

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CD8 Antibody | 38.65 gallery image 5

Published customer image:
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine Peyer's patch and jejenum by immunofluorescence.
Image caption:
Significant increase of CD8+ cells in the ileum of infected lambs. Ileal Peyer's patches sections from a pair of 6 day-old matched lambs, control (A) and inoculated (B), were labelled with Ab against CD3 (red), CD8 (green) and γδTCR (blue). Increased numbers of yellow CD3+/CD8+ cells (arrow) in the lamina propria (lp) and dome (d) were seen in the inoculated animals compared with the controls. Few or no CD3+/CD8+/γδTCR+ cells were observed. Light blue dots (arrowhead) were identified as autofluorescence and thus differentiated from the specific labelling which was localised in the cell membrane. Absorptive epithelium (ae), follicle-associated epithelium (fae), follicle (f), interfollicular area (ifa). Bar 50 μm. (C) The increase of CD3+/CD8+ cells in the ileal Peyer's patch was demonstrated by quantitative analysis of positive cells per mm2 in villi and dome, including the covering epithelium. The graphs show the median with 95% confidence interval constructed using the Bernoulli-Wilcoxon procedure. Statistically significant differences are indicated as ** p?< 0.01 and *** p?<?0.001.

From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment".
Veterinary Research 2015 46:28.

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CD8 Antibody | 38.65 gallery image 6

Published customer image:
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine jejenum by flow cytometry.
Image caption:
Perforin+?lymphocytes among CD8+ lymphocytes in the gut.(A) Cells extracted from gut tissues were double labelled with anti NCR1 and CD8 mAbs then fixed and permeabilized and labelled with an anti perforin mAb. The plot shows (example of jejunum at 6 dpi) the gating of the lymphocyte populations analyzed. (B) Lymphocytes from an inoculated lamb (3 dpi) and its control were gated either on the CD8+/NCR1+ or the CD8tot/NCR1- populations then analyzed for the perforin content. The red line limited histogram corresponds to the inoculated lamb (In), the black line limited histogram to its matched control (Co) and the gray filled histogram to the isotype control of the perforin mAb (iso) and the mean fluorescence intensity (MFI) is indicated for each histogram. (C) The analysis of the perforin expression by CD8+ lymphocyte sub-populations (gates shown on Figure 7A) was performed in 3 control and 4 age-matched 6 dpi inoculated lambs. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p?<?0.01 and *** p?< 0.001.

From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment".
Veterinary Research 2015 46:28.

Enlarge
CD8 Antibody | 38.65 gallery image 7

Published customer image:
Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine jejenum by flow cytometry.
Image caption:
Expression of CD25 on small intestinal NCR1+ and CD8+ lymphocytes. (A) The expression of the activation marker CD25 was analyzed at 6 dpi in the populations gated as indicated. The individual percentages (B) and the mean fluorescence intensity (MFI) of CD25+ cells (C) are shown for the 3 populations. Medians are shown with red bars. Control lambs (open symbols), inoculated lambs (filled symbols). In C, the results of the two experiments are shown with red or black symbols. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p?<?0.01 and *** p?<?0.001.

From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment".
Veterinary Research 2015 46:28.

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  • Mouse anti Sheep CD8:RPE
  • Mouse anti Sheep CD8:FITC
  • Mouse anti Sheep CD8
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    38.65
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2216FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2216F
    MCA2216GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2216GA
    MCA2216PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2216PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.

      Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Ovine efferent lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Antibody : RPE 38.65 F 100 Tests
    CD8 Antibody : Purified 38.65 C, F, IP 0.1 mg
    CD8 Antibody : FITC 38.65 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:RPEMCA929PE100 TestsF
        MCA929PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Sheep CD4:Alexa Fluor® 647MCA2213A647100 Tests/1mlF
          MCA2213A647
          Mouse anti Sheep CD4:FITCMCA2213F0.1 mgF
          MCA2213F
          Mouse anti Sheep CD4:RPEMCA2213PE100 TestsF
          MCA2213PE

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen

              References

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