CD45 Antibody | VPM18

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CD45 Antibody | VPM18 gallery image 1

Sheep peripheral blood lymphocytes stained with Mouse anti Sheep CD45 (MCA896GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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  • Product Type
    Monoclonal Antibody
  • Clone
    VPM18
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA896GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA896GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Sheep CD45, clone VPM18 recognizes the ovine homologue of human CD45 otherwise known as LCA (leucocyte common antigen), a type I transmembrane glycoprotein and member of the protein-tyrosine phosphatase (PTP) family, expressed at high levels on all nucleated cells of haematopoietic origin, and which exists in several isoforms generated by the alternative splicing.

      CD45 is a signal transduction protein vital for T and B cell antigen receptor-mediated activation, and is involved in many aspects of cellular processes, including growth, differentiation and oncogenic transformation. Studies have reported associations between CD45 and many cell surface antigens, including CD2, CD3, CD4, CD26 and CD90.

      Clone VPM18 recognizes all isoforms of the CD45 molecule.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanno
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Sheep T lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45 Antibody Formats

    Formats Clone Applications Sizes available
    CD45 Antibody : Purified VPM18 C, F, IP 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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