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FITC conjugated Mouse anti Ovine CD44 antibody, clone 25.32 (MCA2219F) used for the evaluation of CD44 expression on bone marrow derived ovine mesenchymal stem cells by flow cytometry.
Morphologic and phenotypic characterization of oBMSCs.
(A) Image of ovine bone marrow mesenchymal stromal cells at passage 5 and 8 isolated from iliac crest aspirates. (B) Image of human bone marrow mesenchymal stromal cells (hBMSCs) at passage 10 isolated from bone marrow aspirates, as previously described [Sanjurjo-Rodríguez 2014]. Both images show the typical spindle-shaped fibroblast-like morphology characteristic of the MSC. Original magnification x100. (C) Phenotypic characterization by flow cytometry of a representative population of oBMSCs, for markers characteristic of MSCs and hematopoietic cells that react with sheep. The purple line signifies the specific antibody, while the orange line represents the isotype control. Plots correspond to a representative experiment (D) Bar graph representing the percentage average values (mean ± standard error) of positivity of the markers analyzed from 3 samples at different passages (at 3rd, 4th and 8th passages); markers characteristic of MSCs (CD29, CD44, CD166), embryonic cells (SSEA-4), and hematopoietic cells (CD45).
From: Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, Fernández-Vázquez T, Fuentes-Boquete IM, de Toro-Santos FJ, et al. (2017)
Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.
PLoS ONE 12(1): e0171231.