CD335 Antibody | EC1.1

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CD335 Antibody | EC1.1 gallery image 1

Immunostaining of paraffin embedded, zinc-fixed ovine paracortex medial iliac lymph node for CD335 (NKp46) using Mouse anti Sheep CD335 (MCA5933GA) with a biotin based detection system followed by tryamide signal amplification (image courtesy of Prof. Gary Entrican and Laura Doull, Moredun Research Institute)

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CD335 Antibody | EC1.1 gallery image 2

Immunostaining of paraffin embedded, zinc-fixed ovine medial iliac lymph node for CD335 (NKp46) using Mouse anti Sheep CD335 (MCA5933GA) with a biotin based detection system followed by tryamide signal amplification (image courtesy of Prof. Gary Entrican and Laura Doull, Moredun Research Institute)

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CD335 Antibody | EC1.1 gallery image 3

Sheep CD335 transfected cells stained with Mouse anti Sheep CD335 (MCA5933GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD335 Antibody | EC1.1 gallery image 4

Sheep CD335 transfected cells stained with Mouse anti Sheep CD335: AlexaFluor®488 (MCA5933A488)

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CD335 Antibody | EC1.1 gallery image 5

Sheep CD335 transfected cells stained with Mouse anti Sheep CD335:RPE (MCA5933PE)

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  • Mouse anti Sheep CD335:RPE
  • Mouse anti Sheep CD335
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    EC1.1
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5933GAC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA5933GA
    MCA5933PEFdatasheet pdfdatasheet pdf100 Tests
    MCA5933PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Sheep CD335, clone EC1.1, recognizes the ovine homologue of human CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1.

      CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which function as an activating receptor.

      Research has indicated that, in mammals, the CD335 antigen is confined to NK cells (Westergard et. al. 2004) and that it is primarily expressed by a subset of natural killer (NK) cells which are CD3-ve, CD4-ve and CD2+ve with a small population also being CD8+ve (Storset et. al. 2004). No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.

      NK cells are cytotoxic and form a key component of the innate immune system where they are involved in mediating immunity to tumours and pathogens and in particular are critical to the process where the host recognizes and rejects tumour and viral infected cells.

      Clone EC1.1 (originally named as Gr13.1) is the first antibody specifically raised to ovine CD335 and provides a reagent enabling researchers to identify ovine NKp46 positive NK cells and may facilitate an increased understanding of the ovine immune system and in particular its role in host pathogen defence (Connelley et. al. 2011).

      In addition to clone EC1.1, clones AKS1, which recognises CD335 (NKp46) in bovine and other ruminants, and VIV-KM1, which recognises porcine CD335, are also available from Bio-Rad.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
    • Immunogen
      OvNKp46-mFcγ2b fusion protein
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the X63 myeloma cell line
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution
      18 months from date of despatch
    • GO Terms
      integral to membrane
      plasma membrane
      receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      The Renaissance TSA Biotin system is a registered trademark of PerkinElmer, Waltham, USA
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      Clone EC1.1 is suitable for use on paraffin sections when fixed with zinc salts This product requires antigen retrieval using Renaissance TSA Biotin system®

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to their test reagent.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD335 Antibody Formats

    Formats Clone Applications Sizes available
    CD335 Antibody : Purified EC1.1 C, F, P * 0.1 mg
    CD335 Antibody : RPE EC1.1 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat anti Human CD3:Alexa Fluor®488MCA1477A488100 Tests/1mlF *
          MCA1477A488
          Rat anti Human CD3:Alexa Fluor®647MCA1477A647100 Tests/1mlF *
          MCA1477A647
          Mouse anti Bovine CD205:FITCMCA1651F0.1 mgF
          MCA1651F
          Mouse anti Human CD16:FITCMCA5665F0.1 mgF
          MCA5665F
          Rat anti Human CD3:Pacific Blue®MCA1477PB100 Tests/1mlF *
          MCA1477PB
          Mouse anti Human CD16MCA5665GA0.1 mgF, FN*
          MCA5665GA
          Mouse anti Sheep CD16-LikeMCA919GA0.1 mgC, E, F, IP
          MCA919GA
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat anti Human CD3:FITCMCA1477F0.1 mgF *
          MCA1477F
          Mouse anti Bovine CD205:FITCMCA1651F0.1 mgF
          MCA1651F
          Mouse anti Human CD16:FITCMCA5665F0.1 mgF
          MCA5665F
          Rat anti Human CD3:Pacific Blue®MCA1477PB100 Tests/1mlF *
          MCA1477PB
          Mouse anti Bovine CD205MCA1651GA0.1 mgC, F, IP
          MCA1651GA
          Mouse anti Human CD16MCA5665GA0.1 mgF, FN*
          MCA5665GA
          Mouse anti Sheep CD16-LikeMCA919GA0.1 mgC, E, F, IP
          MCA919GA

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

              Further Reading

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