CD25 Antibody | 9.14

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CD25 Antibody | 9.14 gallery image 1

Staining of ovine peripheral blood lymphocytes with Mouse anti Sheep CD25 (MCA2218GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F). Ovine blood was stimulated for 48 hours with 10 µg/ml LPS and 10 µg/ml PHA prior to staining

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  • CD25 Antibody | 9.14 thumbnail image 1
  • Mouse anti Sheep CD25
  • Mouse anti Sheep CD25:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    9.14
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2218GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2218GA
    MCA2218FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2218F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti sheep CD25, clone 9.14, is specific for the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Rα), a single pass type I membrane protein expressed by activated ovine T-cells.

      Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132). A non-covalent association of the α and β subunits is required to form the high affinity receptor for IL-2.

      Immunoprecipitation studies have demonstrated that clone 9.14 precipitates a band of approximately 47 kDa under reducing conditions, which is that expected of the mature protein due to high glycosylation, and is consistent with the observed molecular weight of IL-2Rα in other species such as humans (Verhagen, A. M., et. al.1993).

      Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi, M.S,. et. al 2011).

      Clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25 and may be of used in facilitating further studies of T-regs in this species.
    • Intended Use
    • Target Species
      Sheep
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch.
    • GO Terms
      receptor activity
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : FITC 9.14 F 0.1 mg
    CD25 Antibody : Purified 9.14 C, F, IP 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Further Reading

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