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14-3-3 Epsilon Antibody

14-3-3 Epsilon Antibody gallery image 1

Western blots of 14-3-3 isoforms in HEK293 cell extracts using Bio-Rad antibodies.
Lane 1 = anti-gamma 14-3-3 (AHP1047)
Lane 2 = anti-zeta 14-3-3 (AHP1052)
Lane 3 = anti-C-epsilon-14-3-3 (AHP1045)
Lane 4 = anti-N-epsilon-14-3-3 (AHP1048)
Lane 5 = anti-eta 14-3-3. (AHP1046)
The 14-3-3 isoforms run at 30 kDa except epsilon 14-3-3 which migrates at 33 kDa

  • 14-3-3 Epsilon Antibody thumbnail image 1
  • Rabbit anti 14-3-3 Epsilon (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1045E, P, WBdatasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti 14-3-3 antiubody recognizes a C-terminal epitope of 14-3-3 epsilon, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.

      14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G checkpoint (Aitken, A. et al. 2006). During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MammalsExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to sheep anti 14-3-3 epsilon C-terminus were raised by repeated immunisations rabbit of with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Synthetic corresponding to C-terminal sequence of sheep 14-3-3 epsilon.

      Peptide sequence: CGEEQNKEALQDVEDENQ
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein domain specific binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Western Blotting1/3000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Normal brain tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti 14-3-3 epsilon antibody detects a band of ~30kDa in HEK293 cell lysates.
    • Instructions For Use

    Additional 14-3-3 Epsilon Antibody Formats

    Formats Applications Sizes available
    14-3-3 Epsilon Antibody : Serum E, P, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Rabbit anti 14-3-3 Isoform PanelPAN01725 µl X 7E *, P *, WB*

          Recommended Positive Controls

            Histology Controls

              Normal brain tissue
                • Product Images

                • 14-3-3 Epsilon Antibody thumbnail image 1


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