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Mouse Serum

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Mouse serum (C11SA) used as a component of the wash buffer prior to incubation with anti mouse secondary antibody to reduce any non specific binsing of the anti mouse immunoglobulin during flow cytometric analysis of cell surface primary mouse antibody binding.
Image caption:
β cells are antigenically heterogeneous in normal and pathological islets.
Human islet samples were enzymatically dispersed and antibody labelled for flow cytometric analysis, and β cells were isolated by FACS with the sorting scheme illustrated in . (a,b) Representative examples of CD9 versus ST8SIA1 expression on the purified β cell populations from two healthy islet donors. (c–j) CD9 versus ST8SIA1 expression on cells from eight islet samples collected from T2D patients. (k) Mean β cell subtype distribution (±s.d.) from 17 normal islet preps. Significant frequency variance (P<1 × 10−24, single factor analysis of variance) was observed between these populations and β1 is significantly more frequent (P=8 × 10−4, 1.2 × 10−12, 5 × 10−13 by t-test (unequal variance)) than any of the others and β2 was significantly more frequent than β3 or β4 (1 × 10−8, 2 × 10−9 by t-test (unequal variance)) as indicated with asterisks. (l) The mean percentage (±s.d.) of ST8SIA1+ β cells is abnormally high (P=0.028, t-test (unequal variance)) in T2D islets (n=8) compared with normal islets (n=17).

From: Dorrell C, Schug J, Canaday PS, Russ HA, Tarlow BD, Grompe MT, Horton T, Hebrok M, Streeter PR, Kaestner KH, Grompe M.
Human islets contain four distinct subtypes of β cells.
Nat Commun. 2016 Jul 11;7:11756.

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Mouse Serum

Product Type
Serum
Specificity
Mouse Serum

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C11SD
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C IY P 100 ml
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Product Form
Whole serum - liquid
Preparation
Normal mouse serum, 0.45um filtered. Prepared from barrier mice which are screened for infectious agents.
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoassay
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Mouse Serum

  1. Dorrell, C. et al. (2016) Human islets contain four distinct subtypes of β cells.
    Nat Commun. 7: 11756.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"