Secondary Antibodies and Reagents for Research

Full List of Secondary Antibodies

Our secondary antibodies table lists every secondary antibodies in one location. You can filter by conjugates, target species and more

View our full list of secondary antibodies

A secondary antibody is used to detect an unconjugated primary antibody that has bound to a target antigen. Secondary antibodies conjugated to enzymes and labels are key components of detection systems – selection of an optimum secondary antibody can improve staining and reduce false positive or negative staining.

Primary Antibodies

Bio-Rad manufacturers and distributes  high quality immunological reagents with almost thirty years of experience, and a portfolio of primary antibodies (monoclonal and polyclonal) and related products. 

View our range of primary antibodies.

Bio-Rad’s range of secondary reagents has been carefully selected to provide optimum quality and flexibility. Secondary antibodies are available in many formats and are useful in a wide range of applications, including flow cytometry (FITC & RPE), immunocytochemistry (HRP & alkaline phosphatase) and Western blotting (HRP & Biotin).

There are a number of reasons why you may need to use a secondary antibody, including:

  • If there is no conjugated primary antibody available.
  • Your primary antibody isn’t conjugated to the required fluorochrome/enzyme.
  • You need to increase the sensitivity of your staining procedure.

Our Recommended Secondary Antibodies

For most secondaries, we offer a choice of monoclonal or polyclonal antibodies suitable to meet these needs, including whole IgG molecules, or F(ab’)2 fragments.

All secondary reagents in our “STAR” range are affinity-purified, and have been carefully optimized for specificity and titer. Several of our anti-mouse, anti-rat, and anti-hamster IgG reagents have also been carefully adsorbed against rat and mouse immunoglobulins to avoid cross-species reactivity.

Human

Secondary antibodies for use with Human antibodies.

Bovine

Secondary antibodies for use with Bovine antibodies.

Canine

Secondary antibodies for use with Canine antibodies.

Caprine

Secondary antibodies for use with Caprine antibodies.

Chicken

Secondary antibodies for use with Chicken antibodies.

Cavia

Secondary antibodies for use with Cavia antibodies.

Equine

Secondary antibodies for use with Equine antibodies.

Feline

Secondary antibodies for use with Feline antibodies.

Hamster

Secondary antibodies for use with Porcine antibodies.

Monkey

Secondary antibodies for use with Porcine antibodies.

Porcine

Secondary antibodies for use with Porcine antibodies.

Rabbit

Secondary antibodies for use with Rabbit antibodies.

Sheep

Secondary antibodies for use with Sheep antibodies.

Mouse

Secondary antibodies for use with Mouse antibodies.

Rat

Secondary antibodies for use with Rat antibodies.


Full List of Secondary Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    Things To Consider When Selecting The Secondary Antibody

    Choosing the correct secondary antibody is critical for obtaining precise results. It is important that the following is taken into account:

    1. The Host That the Primary Antibody Was Developed In
      For immunohistochemistry, the primary antibody should be raised in a species as phylogenetically different as possible to the species of your sample, to avoid cross-reactivity of the secondary antibody.
    2. Your Application Will Affect Your Label
      I.e. for ELISA and immunoblotting, enzyme-linked secondaries are the most popular choice, whereas for flow cytometry you need to choose a secondary with a fluorochrome attached. For cell or tissue staining, either choose HRP, alkaline phosphatase, or a fluorescent-linked secondary antibody.
    3. Class/Subclass of Antibody
      This is mainly important for monoclonal antibodies, where the secondary antibody should match the class/subclass of the primary antibody. Since polyclonal antibodies (e.g. goat, rabbit, sheep, donkey) are usually IgG class immunoglobulins, anti-IgG secondary antibodies may be used.
    4. Is An Adsorbed Secondary Antibody Available?
      This will reduce non-specific background staining, which can be very important when working with mouse and rat tissues. Beware when working with closely homologous species.
    5. Is a F(ab’)2 Fragment Necessary?
      When working with some immune tissues or cells that contain a lot of Fc receptors, it helps to choose a F(ab’)2 fragment to eliminate non-specific binding. Alternatively, block Fc receptors via an absorption step, using purified IgG from the host species of your secondary antibody.