Rituximab Antibody | AbD18423_hIgG1

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Rituximab Antibody | AbD18423_hIgG1 gallery image 1

Rituximab anti drug antibody (ADA) ELISA using Rituximab (at 1µg/ml - black line or 0.5µg/ml - red line) as the coating reagent followed by human anti Rituximab (HCA201) in 10% human serum followed by HRP conjugated Rituximab as the detection reagent

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HCA201 specificity ELISA using various antigens as coating reagents. HRP conjugated Human anti Rituximab (HCA201) was used as the detection reagent

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  • Human anti Rituximab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-rituximab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical method development. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18423_hIgG1
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA201Edatasheet pdfdatasheet pdf0.1 mg
      HCA201
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. Clone AbD18423_hIgG1 is an anti-idiotypic antibody that specifically recognises the rituximab humanised monoclonal antibody. This fully human antibody can be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Rituximab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP in a sandwich ELISA .
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Rituximab Antibody Formats

      Formats Clone Applications Sizes available
      Rituximab Antibody : Purified AbD18423_hIgG1 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human IgG1 LambdaHCA0490.1 mgE
          HCA049

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            MCA2260F
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Rat anti Rituximab:HRPMCA2260P0.1 mgE
            MCA2260P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Human anti RituximabHCA1860.1 mgE
            HCA186
            Rat anti RituximabMCA22600.2 mgE, F
            MCA2260

            Recommended Positive Controls

              Histology Controls

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