Rituximab Antibody | AbD18423_hIgG1

Rituximab Antibody | AbD18423_hIgG1 gallery image 1

Rituximab anti drug antibody (ADA) ELISA using Rituximab (at 1µg/ml - black line or 0.5µg/ml - red line) as the coating reagent followed by human anti Rituximab (HCA201) in 10% human serum followed by HRP conjugated Rituximab as the detection reagent

Rituximab Antibody | AbD18423_hIgG1 gallery image 2

HCA201 specificity ELISA using various antigens as coating reagents. HRP conjugated Human anti Rituximab (HCA201) was used as the detection reagent

Rituximab Antibody | AbD18423_hIgG1 gallery image 3

Figure A. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) (red) or Human Fab-FH isotype control (HCA045) (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) (red) or Human Fab-FH isotype control (HCA045) (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix. Note that the HCA201 is a full IgG1 format of the same Fab format antibody, clone AbD18423 used in this experiment.

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  • Human anti Rituximab
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    Anti-rituximab antibody is a recombinant anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical method development. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA201Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. Clone AbD18423_hIgG1 is an anti-idiotypic antibody that specifically recognises the rituximab humanised monoclonal antibody. This fully human antibody can be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP in a sandwich ELISA .
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Rituximab Antibody Formats

      Formats Clone Applications Sizes available
      Rituximab Antibody : Purified AbD18423_hIgG1 E 0.1 mg
      • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human IgG1 LambdaHCA0490.1 mgE

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            Human anti Rituximab:HRPHCA061P0.1 mgE
            Human anti Rituximab:HRPHCA062P0.1 mgE
            Rat anti Rituximab:HRPMCA2260P0.1 mgE
            Human anti RituximabHCA0610.1 mgE
            Human anti RituximabHCA0620.1 mgE
            Human anti RituximabHCA1860.1 mgE
            Rat anti RituximabMCA22600.2 mgE, F

            Recommended Positive Controls

              Histology Controls

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