IgG Antibody | 8718

//www.bio-rad-antibodies.com/rhesus-monkey-igg-antibody-8718-hca132.html?RenderPageSpecific=1
IgG Antibody | 8718 gallery image 1

Detection of rhesus monkey IgG (PmoP001) and human IgG with Human anti Rhesus Monkey IgG (HCA132) followed by Mouse anti Histidine Tag:HRP (MCA5995P)

Enlarge
  • IgG Antibody | 8718 thumbnail image 1
  • Human anti Rhesus Monkey IgG
  • Human anti Rhesus Monkey IgG
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    8718
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA132Edatasheet pdfdatasheet pdf0.1 mg
    HCA132
    HCA132AEdatasheet pdfdatasheet pdf50 µg
    HCA132A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Rhesus Monkey IgG antibody, clone 8718 specifically recognizes Rhesus Monkey (Macaca mulatta) IgG.

      Tests indicate that clone 8718 and clone 8717 recognize a similar or identical epitope.

      Clone 8718 has been tested against mouse, rat, sheep, goat and human IgG and shows minimal cross reactivity.

    • Intended Use
    • Target Species
      Rhesus Monkey
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      metal chelate affinity chromatography
      metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Purified Rhesus monkey IgG
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/501/100
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/501/100

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product is suitable for use in indirect ELISA applications. PMOP001 may be used as a standard.

      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
      This product is suitable for use in indirect ELISA applications. PMOP001 may be used as a standard.
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Clone Applications Sizes available
    IgG Antibody : Purified 8718 E 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049B125 mlIY
          BUF049B
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          Hispec Assay DiluentBUF049C500 mlIY
          BUF049C
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049B125 mlIY
          BUF049B
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          Hispec Assay DiluentBUF049C500 mlIY
          BUF049C

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...