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Red Fluorescent Protein antibody

Rabbit anti Red Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Red Fluorescent Protein

Product Code Applications Pack Size List Price Your Price Qty
AHP2987
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µg loader
List Price Your Price
loader

Rabbit anti red fluorescent protein antibody recognizes the red fluorescent protein DsRed isolated from the mushroom coral Discosoma striata. The protein is also known as RFP and dsdrFP583 (Day and Davidson 2009). DsRed is a ~27 kDa protein that is optimally excited at a wavelength of 558 nm and emits light at 583 nm (Day and Davidson 2009). In contrast to most other fluorescent proteins, which are monomers, DsRed tends to appear in a tetramer configuration.

Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to red fluorescent protein (RFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
5mM EDTA
0.03% ProClin 300
Immunogen
E. coli expressed recombinant red fluorescent protein
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20°C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti RFP antibody recognizes a band of approximately 27 kDa on recombinant RFP. The observed band size is approximately 32 kDa on SDS PAGE gel.

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Further Reading

  1. Day, R.N. & Davidson, M.W. (2009) The fluorescent protein palette: tools for cellular imaging.
    Chem Soc Rev. 38 (10): 2887-921.

Synonyms
DsRed
RFP

AHP2987

159240 180813

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