HuCAL antibodies technical manual with application protocols

  • Introduction to recombinant antibodies
  • HuCAL technology
  • Applications: ELISA
  • Applications: Flow cytometry
  • Applications: Immunofluorescence
  • Antibody conjugation
  • Choosing the best HuCAL antibody format
  • Reagents to support HuCAL assay development


How to get technical support

An experienced group of technical advisors is available to assist with questions regarding custom HuCAL monoclonal antibodies and their use in a wide range of applications.

Contact us

For help with catalog antibodies and secondary reagents, contact your local Bio-Rad technical support team


References

Publications citing the use of HuCAL antibodies by our customers, and also relating to the development of the HuCAL technology.



HuCAL antibody formats and tags explained

Information about monovalent and bivalent Fab formats and conversion to full immunoglobulin format with a choice of isotypes.



Recommended secondary reagents for detection tags by application

 

C

E

F

IF

IP

P

WB

Catalog Number

Anti-human IgG F(ab’)2:AP

 

 

 

 

 

 

STAR126A

Anti-human IgG F(ab’)2:B.

 

 

 

 

 

STAR126B

Anti-human IgG F(ab’)2:FITC

 

 

 

 

 

STAR126F

Anti-human IgG F(ab’)2:HRP

 

 

 

 

 

STAR126P

Anti-human IgG F(ab’)2:TRITC

 

 

 

 

 

STAR126TRC

Anti-Strep-tag Classic:HRP

 

 

 

 

 

MCA2489P

Anti-Strep-tag Immo (for Fab immobilization)

 

 

 

 

 

 

MCA2488

Anti-Penta-His:HRP

 

 

 

 

 

 

MCA5995P

Anti-His-6

 

 

 

 

 

 

MCA1396

Anti-His-6 tag:A488

 

 

 

 

 

 

 

MCA1396A488

Anti-His-6 tag:A647

 

 

 

 

 

 

 

MCA1396A647

Anti-His-6:FITC

 

 

 

 

 

 

MCA1396F

Anti-His-6:HRP

 

 

 

 

 

MCA1396P

Anti-V5 tag

 

 

 

 

MCA1360

Anti-V5 tag:A647

 

 

 

 

 

 

MCA1360A647

Anti-V5:HRP

 

 

 

 

 

MCA1360P

Anti-V5:FITC

 

 

 

 

 

 

MCA2894F

Anti-V5:FITC

 

 

 

 

 

MCA2895F

Anti-bacterial AP:HRP
(available July 2017)

 

 

 

 

 

HCA275P

Anti-DYKDDDDK:FITC

 

 

 

 

 

 

MCA4764F

Anti-DYKDDDDK:HRP

 

 

 

 

MCA4764P

Anti-DYKDDDDK:HRP

 

 

 

 

 

AHP1074P

Anti-c-myc:HRP

 

 

 

MCA2200P

Anti-mouse IgG:B.

 

 

 

 

 

STAR11B

Anti-human IgG (H+L):PE F(ab’)2 fragment

 

 

 

 

 

 

STAR97PE

Anti-mouse IgG (H+L):PE F(ab’)2 fragment

 

 

 

 

 

 

STAR105PE

Anti-mouse IgG:FITC F(ab’)2 fragment

 

 

 

 

 

 

STAR9B

TidyBlot™ western blot detection reagent:HRP

 

 

 

 

 

 

STAR209P

Notes: Axxx, Alexa Fluor; B., Biotin; C, immunohistology frozen sections; E, ELISA; FC, flow cytometry; IF, immunofluorescence; IP, immunoprecipitation; P, immunohistology paraffin sections, WB, western blot; anti-c-myc antibodies are not recommended for use in P.