Ics Perm/Wash Buffer
|ICS Perm/Wash Buffer allows access to intracellular targets such as cytokines and cytoplasmic proteins which require permeabilization of the cell membrane after fixation. This permeabilization buffer has minimal effect on cell morphology and is supplied as a 10x solution for dilution in water. It can also be used as a wash buffer during intracellular staining and to dilute antibodies during intracellular staining to help minimize background staining. ICS Perm/Wash buffer can also be used as a wash buffer during intracellular staining and to dilute antibodies during intracellular staining to help reduce background staining.|
- Product Form
- 10x concentrate - liquid
- For research purposes only
- Guaranteed until date of expiry. Please see product label.
This product should be stored undiluted.
Crystallization may occur at the reccomended storage conditions, this is normal and does not affect buffer performance. If crystallization remains after dilution to 1X solution, the buffer can be filtered
|Application Name||Verified||Min Dilution||Max Dilution|
- Instructions For Use
- 1. Dilute the ICS Perm/Wash Buffer (10x) to a 1x working concentration using DI water
2. Resuspend fixed cells in ICS Perm/Wash buffer (1x)
3. Centrifuge cells at 350 g for 5-10 min. Repeat twice
4. Stain cells for intracellular targets
It is recomended that the reagent be titrated for optimal performance for each application.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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