HRP-STABILIZING Diluent

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AbGUARD® HRP Stabilizer Plus

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF052A C E P WB 100 ml
BUF052B C E P WB 500 ml
BUF052C C E P WB 1000 ml

Product Details

Intended Use
AbGuard® HRP Stabilizer Plus can be used for the direct dilution and long-term stabilization/storage of Horseradish Peroxidase (HRP)-conjugated proteins and antibodies at both low and high protein concentrations.

HRP-conjugated proteins diluted with AbGuard® HRP Stabilizer Plus can be stored at 4oC for a minimum of 30 months, and can withstand temperatures up to 37oC for 2 weeks or more.

AbGuard® HRP Stabilizer Plus stabilizes the HRP enzyme and optimizes the signal-to-noise ratio in most assays. Bio-Rad recommends the use of our TMB range for this purpose.

Please note - this product cannot be used for the dilution of anti-rabbit immunoglobulin antibodies e.g Goat anti-Rabbit Ig etc.
Product Form
Ready to use - liquid
Preparation
Weakly white liquid based on a Tris buffer with proprietary stabilizing components. Does not contain azide, mercury, BSA or other bovine material. Does contain weak concentrations of rabbit antibodies.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
Please see label for expiry date

More Information

Regulatory
For research purposes only

Applications of HRP-STABILIZING Diluent

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for HRP-STABILIZING Diluent

  1. Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
    Biol Trace Elem Res. 164 (2): 205-11.