Hispec Assay Diluent | N/A
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E ResetHispec Assay Diluent
- Product Type
- Accessory Reagent
- Clone
- N/A
- Specificity
- Hispec Assay Diluent
- Intended Use
- HISPEC assay diluent works to reduce cross reactivity, non-specific binding and matrix effects in immunoassays such as ELISA, EIA, Western blotting, immuno-PCR, protein arrays, multianalyte immunoassays and immunohistochemistry.
The effect of HISPEC assay diluent is dependent on assay system and the antibodies used. HISPEC assay diluent is used instead of a sample buffer or antibody dilution buffer within the immunoassay protocol.
HISPEC assay diluent is unsuitable for blocking of surfaces. For blocking of surfaces we recommend one of the blocking solutions within the Bio-Rad range – see “Useful reagents” below.
Examples of use:
ELISA: use as a dilution buffer for the specimen and for the detection antibodies
Western blotting: use as dilution buffer for primary and secondary antibodies
Immunohistochemistry: use as dilution buffer for primary and secondary antibodies
Protein arrays: use as dilution buffer for specimen and for the detection antibodies - Product Form
- Ready to use assay diluent buffer.
- Preservative Stabilisers
- 0.1% ProClin 300
- Regulatory
- For research purposes only.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunoassay |
- Instructions For Use
-
BUF049C, BUF049B, BUF049A
The buffer should be mixed thoroughly immediately before use.
Dilution of the samples:
The standards and samples for ELISA and protein arrays can be diluted with HISPEC assay diluent at 1:2 or higher. Standards and samples should be treated identically.
Dilution of antibodies:
Antibodies can be diluted with HISPEC assay diluent in a user-defined manner, depending on the recommendation of the antibody supplier.
Expected results:
In some cases a reduction in assay signal intensity may be observed when using HISPEC assay diluent. This does not result in a reduction in assay sensitivity due to the accompanying reduction in non-specific binding.
Signal intensity may be increased in some assay systems by use of higher concentrations of antibodies, or by dilution of the HISPEC assay diluent in distilled water (1:2) prior to use. However, these adjustments may also result in some associated increase in non-specific binding.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
ELISA BSA Block | BUF032A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | ELISA BSA Block | ||||||
ELISA Ultrablock | BUF033A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | ELISA Ultrablock | ||||||
ELISA Synblock | BUF034A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | ELISA Synblock |
References for Hispec Assay Diluent
-
Eriksson, O. et al. (2018) Pancreatic imaging using an antibody fragment targeting the zinc transporter type 8: a direct comparison with radio-iodinated Exendin-4.
Acta Diabetol. 55 (1): 49-57. -
Yang, X. et al. (2022) Exploring the value of Mycobacterium tuberculosis. modified lipoprotein as a potential biomarker for TB detection in children.
BMC Infect Dis. 22 (1): 158. -
Hentrich, C. et al. (2024) Engineered reversible inhibition of SpyCatcher reactivity enables rapid generation of bispecific antibodies.
Nat Commun. 15 (1): 5939.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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