Cell Stimulation Reagent (Without Brefeldin A)


Product Details
- Product Form
- 500x concentrate - liquid
- Preparation
- 500x solution contains: Phorbol-12-myristate-13-acetate (PMA) 40.5 μM and Ionomycin 669.3 μM in DMSO
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of Cell Stimulation Reagent (Without Brefeldin A)
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry |
- Instructions For Use
- 1. Resuspend 1 x106 - 2 x106 cells per ml in cell culture media
2. Thaw the Cell Stimulation Reagent (without Brefeldin A) 500x (BUF076A) in a 37oC water bath
3. To each ml of cell suspension add 2 μl of BUF076A and incubate cells in a CO2, 37oC incubator for 6 hours
4. Harvest cells and centrifuge at 300-400 g for 5 minutes. Discard supernatant
5. To wash the pellet, add 10-20 ml of Cell Staining Buffer (BUF073), vortex to resuspend the pellet then centrifuge at 300-400 g for 5 minutes. Discard supernatant and repeat step 5 for a second time
6. Stain for surface markers or proteins of interest and analyze cells using flow cytometry
Note: Aliquot product to avoid repeat freeze-thawing. Time and culture conditions may need to be optimized. To detect intracellular staining following activation, the Cell Activation Reagent (without Brefeldin A) 500x (BUF076A) can be used with Monensin Solution (BUF074) or Brefeldin A Solution 1000x (BUF075).
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Monensin Solution (1000X) | BUF074 | F | 1 ml |
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Brefeldin A Solution (1000X) | BUF075 | F | 1 ml |
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Cell Stimulation Reagent (with Brefeldin A) (500X) | BUF077A | F | 0.1 ml |
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