Antigen Retrieval Buffer, Universal
- Intended Use
- Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation is suitable for use with most antibodies and gives excellent preservation of tissue morphology.
- Buffer Solution
- Bio-Rad's Antigen unmasking fluid is supplied as 3x concentrated fluid. Crystals in the fluid will dissolve during heating before use.
- For research purposes only
- Guaranteed until date of expiry. Please see product label.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin|
- Instructions For Use
- 1. Deparaffinize sections and re-hydrate.
2. Dilute concentrated Antigen unmasking fluid 1:3 with de-ionised water (1 part Antigen unmasking fluid:2 parts water).
3. Pre-heat retrieval buffer to 92oC *.
4. Place slides in buffer and incubate 10 minutes at 92oC *.
5. Allow to cool for 5-10 minutes.
6. Rinse in distilled water or PBS, proceed with staining.
* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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