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READIDROP™ Propidium Iodide

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Staining of a mixture of live and heat killed Jurkat cells with ReadiDrop Propidium Iodide for 1 min prior to analysis

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  • READIDROP™ Propidium Iodide
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1351101Fdatasheet pdfdatasheet pdf3 X 3ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Propidium Iodide (PI) is a fluorescent dye that binds to DNA and RNA in a stoichiometric ratio. PI is not membrane permeable and therefore requires the compromised membranes of dead cells to bind to DNA. Upon excitation with an appropriate light source, dead cells exhibit higher fluorescence intensity than live cells, thereby enabling clear discrimination of these populations by flow cytometry. Dead cells can then be excluded from further analysis and also sorted from cell populations of interest. The spectral properties (Excitation 535 nm/Emission 617 nm) enable use in both single color and multi color flow experiments. The convenient dropper bottle format removes the manual weighing, pipetting and dilution steps required by other commonly available PI products.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at room temperature
    • Shelf Life
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/2501/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Caution: Wear gloves, safety glasses/face protection, and protective clothing to avoid direct contact of ReadiDrop solutions with skin and eyes. Refer to MSDS for more information.

      1. Resuspend 1 x 106 cells in 500 μl flow cytometry buffer (1x phosphate buffered saline with 3% BSA). The cell concentration is now 2 x 106/ml.

      2. Add one to two drop of ReadiDrop Propidium Iodide to the cell suspension.

      3. Vortex briefly.

      4. Incubate for 1-10 min at room temperature.

      Note: Adding one drop to a 500 μl cell suspension produces a final dye concentration of 1 μg/ml. This is typically sufficient to stain 1 x 106 cells. If one drop is not sufficient for your cell type, adding a second drop may improve staining efficacy. When working with a larger volume of cells, scale ReadiDrop solution amount accordingly.

      5. Analyze cells by flow cytometry.

      - Detect PI signal in FL3 on Bio-Rad's S3e Cell Sorter. Otherwise, select the most appropriate FL channel on your particular instrument.

    Additional READIDROP™ Propidium Iodide Formats

    Formats Applications Sizes available
    READIDROP™ Propidium Iodide : Reagent F 3 X 3ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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