Tyrosine Hydroxylase (pSer31) Antibody

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Tyrosine Hydroxylase (pSer31) Antibody gallery image 1

Western blot of PC-12 cells that show phosphospecific immunolabeling of the ~60 kDaTyrosine kinase protein phosphorylated at at Ser31

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  • Rabbit anti Tyrosine Hydroxylase (pSer31)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP911C, IF, WB*datasheet pdfdatasheet pdf0.1 ml
    AHP911
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti phosphorylated tyrosine hydroxylase antibody resognizes tyrosine hydroxylase, when phosphorylated at serine 31.

      Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines, dopamine (DA), norepinephrine and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.

      Phosphorylation of TH has been shown to occur at several serine residues, including serine 8, 19, 31 and 40.

      Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al. 1979).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/1000
      Immunohistology - Frozen1/1000
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP911 detects a band of approximately 60kDa in rat PC-12 cell lysates, following stimulation by Okadaic acid.
    • Instructions For Use

    Additional Tyrosine Hydroxylase (pSer31) Antibody Formats

    Formats Applications Sizes available
    Tyrosine Hydroxylase (pSer31) Antibody : Purified C, IF, WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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