Tyrosine Hydroxylase Antibody

Tyrosine Hydroxylase Antibody gallery image 1

Western blot of rat caudate lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein

Tyrosine Hydroxylase Antibody gallery image 2

Published customer image:
Rabbit anti tyrosine hydroxylase antibody used to demonstrate tyrosine hydroxylase reactivity in nerve fibers in a rat myocarcial ischemia model by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
TH immunohistochemical staining of the infarct border zone (DAB color, ×200). (a) Sham-operated group. The distribution of TH-positive nerve fibers is orderly. (b) Control ischemic group. TH-positive nerve fibers in ventricular muscle increased significantly it is thickened and disorderly. (c) Preischemic treatment group. (d) Delayed application group. The distribution of nerve fibers after treatment with desipramine significantly improved. The change of preischemic treatment group was more obvious.

From: Wu X, Jiang H, Yu L, Hu X, Liu W.
Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
J Biomed Biotechnol. 2012;2012:732909.

  • Tyrosine Hydroxylase Antibody thumbnail image 1
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  • Rabbit anti Tyrosine Hydroxylase
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP931C, IF, P, WBdatasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Rat tyrosine hydroxylase antibody recognizes tyrosine hydroxylase (TH). Tyrosine hydroxylase catalyses the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.

      Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease (Haavik et al. 1998).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1000
      Immunohistology - Paraffin
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP931 detects a band of approximately 60kDa in rat caudate cell lysates.
    • Instructions For Use

    Additional Tyrosine Hydroxylase Antibody Formats

    Formats Applications Sizes available
    Tyrosine Hydroxylase Antibody : Purified C, IF, P, WB 0.1 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Paraffin


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