Tryptophan Hydroxylase 2 (pSer19) Antibody

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Tryptophan Hydroxylase 2 (pSer19) Antibody gallery image 1

Western blot of recombinant tryptophan hydroxylase with and without CaMKII showing phosphospecific immunolabeling of ~55 kDa protein phosphorylated at Ser12

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  • Rabbit anti Tryptophan Hydroxylase 2 (pSer19)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1307WB*datasheet pdfdatasheet pdf0.1 ml
    AHP1307
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat tryptophan hydroxylase 2 (pSer19) antibody recognizes tryptophan hydroxylase 2, also known as neuronal tryptophan hydroxylase and tryptophan 5-monooxygenase 2, when phosphorylated at serine 19. It does not cross-react with the non-phosphorylated protein.

      Tryptophan hydroxylase (TPH) is a rate-limiting enzyme which catalyses the 5-hydroxylation of tryptophan, to form the amino acid 5-hydroxytryptophan (5-HTP), in the first stage of the biosynthesis of the neurotransmitter serotonin (5-hydroxytryptamine/5HT). Mammalian TPH is encoded by two genes: TPH1 expressed in peripheral tissues e.g. skin and gut, and TPH2 primarily expressed in the brain. Studies have demonstrated the regulation/enhancement of TPH activity through phosphorylation by cAMP−dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaMKII), the latter of which phosphorylates a Ser19 site within the regulatory domain of TPH2.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      ZebrafishExpected from Sequence
      MouseExpected from Sequence
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat tryptophan hydroxylase 2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.01%Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Synthetic phospeptide corresponding to an amino acid sequence within TPH2, which includes the phosphorylated Ser19 residue.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      response to stress
      response to glucocorticoid stimulus
      iron ion binding
      response to estrogen stimulus
      amino acid binding
      oxidation-reduction process
      cellular response to lithium ion
      serotonin biosynthetic process
      circadian rhythm
      tryptophan 5-monooxygenase activity
      response to activity
      response to nutrient levels
      response to calcium ion
      aromatic amino acid family metabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1307 detects a band of approximately 55kDa on lysates from human dorsal Raphe nucleus.
    • Instructions For Use

    Additional Tryptophan Hydroxylase 2 (pSer19) Antibody Formats

    Formats Applications Sizes available
    Tryptophan Hydroxylase 2 (pSer19) Antibody : Purified WB* 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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