TCR V Beta 16 .1 Antibody | HIS42

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  • Mouse anti Rat TCR V Beta 16.1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HIS42
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA472SC *, F, IPdatasheet pdfdatasheet pdf2 ml
    MCA472S
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • detects a minor subset of T cells. The subpopulation of HIS 42 positive T cells contains both CD4 and CD8 positive cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS 42 induces T cell proliferation in the presence of IL-2. In contrast to lymph node T cells a number of thymocytes are found to express HIS 42 only in the cytoplasm or together with membrane expression. Most bright HIS 42 surface labelled thymocytes are also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS PAGE analysis of the membrane molecules immunoprecipitated by HIS 42 shows a band at 85 kDa. On reduction it separates to two bands at 35 kDa and 48 kDa. From this data it is concluded that HIS 42 is directed against a determinant on the rat T cell receptor, which is common to a small number of T cells. Preliminary data suggests that the determinant is found on the beta chain, associated with the Vbeta16.1 gene product (Kampinga et al. 1989).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      PVG RT7.2 Rat Thymocytes.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/10
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TCR V Beta 16 .1 Antibody Formats

    Formats Clone Applications Sizes available
    TCR V Beta 16 .1 Antibody : S/N HIS42 C *, F, IP 2 ml
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

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              References

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